Role of Uric Acid in Vascular Remodeling: Cytoskeleton Changes and Migration in VSMCs.
Mice
Animals
Muscle, Smooth, Vascular
/ metabolism
Uric Acid
/ pharmacology
Vascular Remodeling
Angiotensin Receptor Antagonists
/ pharmacology
Proteasome Endopeptidase Complex
/ metabolism
Angiotensin-Converting Enzyme Inhibitors
/ pharmacology
Cytoskeleton
/ metabolism
Cell Movement
Myocytes, Smooth Muscle
/ metabolism
Cells, Cultured
Cell Proliferation
migration
phenotypic transition
uric acid
vascular remodeling
vascular smooth muscle cell
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
03 Feb 2023
03 Feb 2023
Historique:
received:
08
01
2023
revised:
29
01
2023
accepted:
01
02
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
The mechanisms by which hyperuricemia induces vascular dysfunction and contributes to cardiovascular disease are still debated. Phenotypic transition is a property of vascular smooth muscle cells (VSMCs) involved in organ damage. The aim of this study was to investigate the effects of uric acid (UA) on changes in the VSMC cytoskeleton, cell migration and the signals involved in these processes. MOVAS, a mouse VSMC line, was incubated with 6, 9 and 12 mg/dL of UA, angiotensin receptor blockers (ARBs), proteasome and MEK-inhibitors. Migration property was assessed in a micro-chemotaxis chamber and by phalloidin staining. Changes in cytoskeleton proteins (Smoothelin B (SMTB), alpha-Smooth Muscle Actin (αSMA), Smooth Muscle 22 Alpha (SM22α)), Atrogin-1 and MAPK activation were determined by Western blot, immunostaining and quantitative reverse transcription PCR. UA exposition modified SMT, αSMA and SM22α levels (
Identifiants
pubmed: 36769281
pii: ijms24032960
doi: 10.3390/ijms24032960
pmc: PMC9917405
pii:
doi:
Substances chimiques
Uric Acid
268B43MJ25
Angiotensin Receptor Antagonists
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
Angiotensin-Converting Enzyme Inhibitors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fondazione Cassa di Risparmio di Genova e Imperia
ID : D.R. 3707
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