SARS-CoV-2 Spike Protein Activates Human Lung Macrophages.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
03 Feb 2023
Historique:
received: 16 01 2023
revised: 30 01 2023
accepted: 01 02 2023
entrez: 11 2 2023
pubmed: 12 2 2023
medline: 15 2 2023
Statut: epublish

Résumé

COVID-19 is a viral disease caused by SARS-CoV-2. This disease is characterized primarily, but not exclusively, by respiratory tract inflammation. SARS-CoV-2 infection relies on the binding of spike protein to ACE2 on the host cells. The virus uses the protease TMPRSS2 as an entry activator. Human lung macrophages (HLMs) are the most abundant immune cells in the lung and fulfill a variety of specialized functions mediated by the production of cytokines and chemokines. The aim of this project was to investigate the effects of spike protein on HLM activation and the expression of ACE2 and TMPRSS2 in HLMs. Spike protein induced CXCL8, IL-6, TNF-α, and IL-1β release from HLMs; promoted efficient phagocytosis; and induced dysfunction of intracellular Ca

Identifiants

pubmed: 36769357
pii: ijms24033036
doi: 10.3390/ijms24033036
pmc: PMC9917796
pii:
doi:

Substances chimiques

spike protein, SARS-CoV-2 0
Spike Glycoprotein, Coronavirus 0
Angiotensin-Converting Enzyme 2 EC 3.4.17.23

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : CISI Lab Project (University of Naples Federico II)
ID : Not available
Organisme : TIMING Project (Regione Campania)
ID : Not available
Organisme : Campania Bioscience (Regione Campania)
ID : Not available
Organisme : MIUR PRIN 2017 M8Y MR8_005 and AIRC - MFAG 2020
ID : (ID. project 25123)

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Auteurs

Francesco Palestra (F)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.

Remo Poto (R)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
World Allergy Organization (WAO) Center of Excellence (CoE), 80131 Naples, Italy.

Renato Ciardi (R)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.

Giorgia Opromolla (G)

Translational Medical and Surgical Science, University of Campania Luigi Vanvitelli, 80131 Naples, Italy.

Agnese Secondo (A)

Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples Federico II, 80131 Naples, Italy.

Valentina Tedeschi (V)

Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples Federico II, 80131 Naples, Italy.

Anne Lise Ferrara (AL)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy.

Rosa Maria Di Crescenzo (RM)

Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples Federico II, 80131 Naples, Italy.

Maria Rosaria Galdiero (MR)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
World Allergy Organization (WAO) Center of Excellence (CoE), 80131 Naples, Italy.
Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy.

Leonardo Cristinziano (L)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.

Luca Modestino (L)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.

Gianni Marone (G)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
World Allergy Organization (WAO) Center of Excellence (CoE), 80131 Naples, Italy.
Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy.
Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy.

Alfonso Fiorelli (A)

Translational Medical and Surgical Science, University of Campania Luigi Vanvitelli, 80131 Naples, Italy.

Gilda Varricchi (G)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
World Allergy Organization (WAO) Center of Excellence (CoE), 80131 Naples, Italy.
Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy.
Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy.

Stefania Loffredo (S)

Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy.
World Allergy Organization (WAO) Center of Excellence (CoE), 80131 Naples, Italy.
Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy.
Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy.

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