Gas Chromatography-Mass Spectrometry (GC-MS) Metabolites Analysis in Endometriosis Patients: A Prospective Observational Translational Study.
biomarkers
endometriosis
gas chromatography–mass spectrometry
metabolomics
multivariate analysis
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
24 Jan 2023
24 Jan 2023
Historique:
received:
16
11
2022
revised:
04
01
2023
accepted:
14
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
12
2
2023
Statut:
epublish
Résumé
Endometriosis affects women of reproductive age, and its pathogenesis is still unclear. Typically, it overlaps other similar medical and surgical conditions, determining a delay in early diagnosis. Metabolomics allows studying metabolic changes in different physiological or pathological states to discover new potential biomarkers. We used the gas chromatography-mass spectrometer (GC-MS) to explore metabolic alterations in endometriosis to better understand its pathophysiology and find new biomarkers. Twenty-two serum samples of patients with symptomatic endometriosis and ten without it were collected and subjected to GC-MS analysis. Multivariate and univariate statistical analyses were performed, followed by pathway analysis. Partial least squares discriminant analysis was performed to determine the differences between the two groups ( The metabolomic approach identifies metabolic alterations in women with endometriosis. These findings may improve our understanding of the pathophysiological mechanisms of disease and the discovery of new biomarkers.
Sections du résumé
BACKGROUND
BACKGROUND
Endometriosis affects women of reproductive age, and its pathogenesis is still unclear. Typically, it overlaps other similar medical and surgical conditions, determining a delay in early diagnosis. Metabolomics allows studying metabolic changes in different physiological or pathological states to discover new potential biomarkers. We used the gas chromatography-mass spectrometer (GC-MS) to explore metabolic alterations in endometriosis to better understand its pathophysiology and find new biomarkers.
METHODS
METHODS
Twenty-two serum samples of patients with symptomatic endometriosis and ten without it were collected and subjected to GC-MS analysis. Multivariate and univariate statistical analyses were performed, followed by pathway analysis.
RESULTS
RESULTS
Partial least squares discriminant analysis was performed to determine the differences between the two groups (
CONCLUSIONS
CONCLUSIONS
The metabolomic approach identifies metabolic alterations in women with endometriosis. These findings may improve our understanding of the pathophysiological mechanisms of disease and the discovery of new biomarkers.
Identifiants
pubmed: 36769570
pii: jcm12030922
doi: 10.3390/jcm12030922
pmc: PMC9918082
pii:
doi:
Types de publication
Journal Article
Langues
eng
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Oncotarget. 2018 Feb 22;9(19):14754-14763
pubmed: 29599905
Gynecol Endocrinol. 2019 Jul;35(7):553-558
pubmed: 30909768
Arch Gynecol Obstet. 2013 Jan;287(1):77-82
pubmed: 22930151
BMC Vet Res. 2010 Oct 27;6:47
pubmed: 20979598
Arch Gynecol Obstet. 2005 Jan;271(1):40-5
pubmed: 15243756
Mol Cell Proteomics. 2010 Sep;9(9):2063-75
pubmed: 20811074
Reprod Biomed Online. 2021 Oct;43(4):727-737
pubmed: 34446375
Gynecol Endocrinol. 2022 Sep;38(9):713-720
pubmed: 35971323
Front Immunol. 2020 Feb 21;11:31
pubmed: 32153556
Mol Omics. 2021 Apr 1;17(2):210-229
pubmed: 33598670
J Intern Med. 2010 Jun;267(6):543-60
pubmed: 20210843
Minerva Obstet Gynecol. 2021 Apr;73(2):193-214
pubmed: 33851803
J Assist Reprod Genet. 2015 Dec;32(12):1817-25
pubmed: 26497128
Nature. 2008 Nov 27;456(7221):443
pubmed: 19037294
Immunol Today. 2000 Apr;21(4):192-9
pubmed: 10740243
Clin Chem. 2012 Jan;58(1):139-47
pubmed: 22110018
Metabolomics. 2018;14(4):52
pubmed: 29576760
Hum Reprod Update. 2019 Sep 11;25(5):564-591
pubmed: 31424502
Nature. 2008 Oct 23;455(7216):1054-6
pubmed: 18948945
Fertil Steril. 2015 Nov;104(5):1202-9
pubmed: 26297644
Arch Gynecol Obstet. 2021 Nov;304(5):1221-1231
pubmed: 34448038
Int Immunopharmacol. 2011 Aug;11(8):932-8
pubmed: 21352963
Br J Nutr. 2007 Aug;98(2):237-52
pubmed: 17403271
Front Biosci (Landmark Ed). 2016 Jun 01;21(5):941-8
pubmed: 27100482
Metabolites. 2021 Jul 02;11(7):
pubmed: 34357331
J Clin Invest. 1969 Mar;48(3):584-94
pubmed: 5773094
RSC Adv. 2018 May 24;8(34):19238-19250
pubmed: 35539642
J Pers Med. 2020 Dec 21;10(4):
pubmed: 33371433
Reprod Biomed Online. 2020 Oct;41(4):640-652
pubmed: 32839101
Adv Exp Med Biol. 2021;1310:509-531
pubmed: 33834448
Curr Med Chem. 2011;18(15):2234-9
pubmed: 21517756
Oxid Med Cell Longev. 2018 Mar 21;2018:7924021
pubmed: 29743986
Front Biosci (Landmark Ed). 2015 Jun 01;20(7):1116-43
pubmed: 25961549
Gynecol Endocrinol. 2015;31(8):595-8
pubmed: 26172932
Nat Rev Drug Discov. 2020 Sep;19(9):609-633
pubmed: 32709961
Curr Protoc Mol Biol. 2016 Apr 01;114:30.4.1-30.4.32
pubmed: 27038389
J Biol Chem. 1957 May;226(1):497-509
pubmed: 13428781
J Genet Genomics. 2020 Feb 20;47(2):69-83
pubmed: 32178981
Front Immunol. 2020 Jul 16;11:1497
pubmed: 32765517
Methods Mol Biol. 2006;324:53-74
pubmed: 16761371
Chin J Nat Med. 2016 Aug;14(8):561-81
pubmed: 27608946
Int J Tryptophan Res. 2017 Jun 14;10:1178646917713491
pubmed: 28659716
Food Chem Toxicol. 1983 Aug;21(4):449-52
pubmed: 6684626
Fertil Steril. 2017 Mar;107(3):699-706.e6
pubmed: 28259259
Methods Mol Biol. 2018;1738:133-147
pubmed: 29654587
Cancer Cell. 2020 Feb 10;37(2):147-156
pubmed: 32049045
Int J Mol Sci. 2021 Dec 03;22(23):
pubmed: 34884907
Endocrinol Diabetes Metab. 2022 Jan;5(1):e00300
pubmed: 34787952
Methods Mol Biol. 2015;1277:161-93
pubmed: 25677154
Gynecol Obstet Invest. 2017;82(5):453-461
pubmed: 27820938
Nutr Rev. 2018 Nov 1;76(11):840-856
pubmed: 30124936
Int J Tryptophan Res. 2018 Jan 08;11:1178646917751636
pubmed: 29343967
Invest Ophthalmol Vis Sci. 2011 Apr 22;52(5):2640-8
pubmed: 21212175
Hum Reprod. 2012 Sep;27(9):2698-711
pubmed: 22736326
Pediatr Clin North Am. 2012 Oct;59(5):1039-58
pubmed: 23036243
J Proteome Res. 2007 Feb;6(2):469-79
pubmed: 17269704
Int J Reprod Biomed. 2016 Jan;14(1):1-8
pubmed: 27141542
Cancer Res. 2016 Feb 15;76(4):796-804
pubmed: 26685161
J Steroid Biochem Mol Biol. 2016 May;159:60-9
pubmed: 26921767
Expert Rev Gastroenterol Hepatol. 2012 Apr;6(2):239-51
pubmed: 22375528
Curr Opin Clin Nutr Metab Care. 2018 Jan;21(1):64-70
pubmed: 29211698
Hum Reprod. 2018 Mar 1;33(3):347-352
pubmed: 29206943
Curr Top Behav Neurosci. 2017;31:95-115
pubmed: 27278641
Mol Biosyst. 2017 May 30;13(6):1246
pubmed: 28524204
Am J Reprod Immunol. 2022 Sep;88(3):e13593
pubmed: 35781369
Gynecol Endocrinol. 2014 Nov;30(11):769-73
pubmed: 25144122
Minerva Ginecol. 2017 Oct;69(5):438-439
pubmed: 28545294
Nat Rev Nephrol. 2011 Oct 25;8(1):22-33
pubmed: 22025087
Sci Rep. 2016 May 19;6:26117
pubmed: 27193963
Cell Physiol Biochem. 2015;35(1):364-73
pubmed: 25591777
Obstet Gynecol Clin North Am. 1997 Jun;24(2):235-58
pubmed: 9163765
Med Hypotheses. 2017 Jun;103:10-20
pubmed: 28571791
Eur J Obstet Gynecol Reprod Biol. 2004 Jul 15;115(1):66-74
pubmed: 15223168
Amino Acids. 2021 Dec;53(12):1917-1925
pubmed: 33818628
Mol Biosyst. 2012 Oct 30;8(12):3281-7
pubmed: 23079773
Fertil Steril. 2018 Sep;110(4):767-777.e5
pubmed: 30196975
Am J Reprod Immunol. 2014 Nov;72(5):496-503
pubmed: 24974860
Am J Reprod Immunol. 2011 Jan;65(1):1-10
pubmed: 20584009
Gynecol Endocrinol. 2020 Nov;36(11):1010-1014
pubmed: 32401078
Hum Reprod Update. 2010 Nov-Dec;16(6):651-74
pubmed: 20462942
Int J Mol Sci. 2020 Mar 04;21(5):
pubmed: 32143537
Front Neurol. 2017 Sep 04;8:459
pubmed: 28928712
Int J Womens Health. 2019 Aug 12;11:443-449
pubmed: 31496832
Adv Nutr. 2016 Jul 15;7(4):798S-805S
pubmed: 27422517
Eur J Obstet Gynecol Reprod Biol. 2011 Feb;154(2):215-7
pubmed: 21075501
Diabetes. 1976 Sep;25(9):776-84
pubmed: 955305
Reprod Sci. 2021 Mar;28(3):728-735
pubmed: 33174185
Sci Rep. 2018 Apr 24;8(1):6466
pubmed: 29691425
Biochemistry. 2018 Jun 26;57(25):3433-3444
pubmed: 29648801
Fertil Steril. 2017 Mar;107(3):707-713.e3
pubmed: 28089574
Int J Surg. 2014 Dec;12(12):1495-9
pubmed: 25046131
Fertil Steril. 1998 Dec;70(6):1101-8
pubmed: 9848302
Int Immunopharmacol. 2021 Dec;101(Pt A):108313
pubmed: 34741868
Best Pract Res Clin Obstet Gynaecol. 2018 Jul;50:72-83
pubmed: 29778458
Acta Obstet Gynecol Scand. 2013 Jan;92(1):3-7
pubmed: 23061819
BMC Med. 2021 Dec 9;19(1):313
pubmed: 34879839
Nucleic Acids Res. 2015 Jul 1;43(W1):W251-7
pubmed: 25897128
Gynecol Endocrinol. 2015 Jun;31(6):454-7
pubmed: 26213861
Expert Opin Drug Metab Toxicol. 2016 May;12(5):581-8
pubmed: 27021205
Biomed Res Int. 2013;2013:329058
pubmed: 23984345