Tocilizumab-treated convalescent COVID-19 patients retain the cross-neutralization potential against SARS-CoV-2 variants.

Health sciences Immunology Virology treatment

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
17 Mar 2023
Historique:
received: 29 06 2022
revised: 10 10 2022
accepted: 30 01 2023
pubmed: 14 2 2023
medline: 14 2 2023
entrez: 13 2 2023
Statut: ppublish

Résumé

Although tocilizumab treatment in severe and critical coronavirus disease 2019 (COVID-19) patients has proven its efficacy at the clinical level, there is little evidence supporting the effect of short-term use of interleukin-6 receptor blocking therapy on the B cell sub-populations and the cross-neutralization of SARS-CoV-2 variants in convalescent COVID-19 patients. We performed immunological profiling of 69 tocilizumab-treated and non-treated convalescent COVID-19 patients in total. We observed that SARS-CoV-2-specific IgG1 titers depended on disease severity but not on tocilizumab treatment. The plasma of both treated and non-treated patients infected with the ancestral variant exhibit strong neutralizing activity against the ancestral virus and the Alpha, Beta, and Delta variants of SARS-CoV-2, whereas the Gamma and Omicron viruses were less sensitive to seroneutralization. Overall, we observed that, despite the clinical benefits of short-term tocilizumab therapy in modifying the cytokine storm associated with COVID-19 infections, there were no modifications in the robustness of B cell and IgG responses to Spike antigens.

Identifiants

pubmed: 36776936
doi: 10.1016/j.isci.2023.106124
pii: S2589-0042(23)00201-8
pmc: PMC9894676
doi:

Types de publication

Journal Article

Langues

eng

Pagination

106124

Subventions

Organisme : NIAID NIH HHS
ID : U01 AI151758
Pays : United States

Informations de copyright

© 2023 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

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Auteurs

Camille Chauvin (C)

Institut Pasteur, Université de Paris, Functional Genetics of Infectious Diseases Unit, Department of Global Health, 75015 Paris, France.
Centre National de la Recherche Scientifique (CNRS), UMR2000, Paris Cedex 15, France.

Laurine Levillayer (L)

Institut Pasteur, Université de Paris, Functional Genetics of Infectious Diseases Unit, Department of Global Health, 75015 Paris, France.
Centre National de la Recherche Scientifique (CNRS), UMR2000, Paris Cedex 15, France.

Mathilde Roumier (M)

Service de Médecine Interne, Hôpital Foch, 92151 Suresnes, France.

Hubert Nielly (H)

Service de Médecine Interne, Hôpital d'Instruction des Armées Bégin, 94160 Saint Mandé, France.

Claude Roth (C)

Institut Pasteur, Université de Paris, Functional Genetics of Infectious Diseases Unit, Department of Global Health, 75015 Paris, France.
Centre National de la Recherche Scientifique (CNRS), UMR2000, Paris Cedex 15, France.

Anupama Karnam (A)

Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, 75006, France.

Srinivasa Reddy Bonam (SR)

Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, 75006, France.

Anne Bourgarit (A)

Hôpital Jean Verdier, HUPSSD, AP-HP, 93140 Bondy, France.
Sorbonne Paris-Nord University (Paris 13), 93000 Bobigny, France.
Inserm, UMR 1135 CIMI, 75013 Paris, France.

Clément Dubost (C)

Service de réanimation, Hôpital militaire Bégin, 94120 Saint Mandé, France.
Université Paris-Saclay, ENS Paris-Saclay, CNRS, Centre Borelli, 91190, Gif-sur-Yvette, France.

Aurore Bousquet (A)

Département des laboratoires, Hôpital militaire Bégin, 94120 Saint Mandé, France.

Sébastien Le Burel (S)

Service de Médecine Interne, Hôpital d'Instruction des Armées Bégin, 94160 Saint Mandé, France.

Raphaële Mestiri (R)

Service de Médecine Interne, Hôpital d'Instruction des Armées Bégin, 94160 Saint Mandé, France.

Damien Sene (D)

Département de médecine interne, Hôpital Lariboisière, Université de Paris (Diderot), AP-HP, 75010 Paris, France.

Joris Galland (J)

Département de médecine interne, Hôpital Lariboisière, Université de Paris (Diderot), AP-HP, 75010 Paris, France.

Marc Vasse (M)

Laboratoire de Biologie Médicale, Hôpital Foch, 92151 Suresnes, France.
UMRS-1176, Le Kremlin Bicêtre, France.

Matthieu Groh (M)

Service de Médecine Interne, Hôpital Foch, 92151 Suresnes, France.

Mathilde Le Marchand (M)

Department of Clinical Research and Innovation, Foch Hospital, 40 rue Worth, 92150 Suresnes, France.

Camille Vassord-Dang (C)

Department of Clinical Research and Innovation, Foch Hospital, 40 rue Worth, 92150 Suresnes, France.

Jean-François Gautier (JF)

Departement of Diabetes and Endocrinology, Hôpital Lariboisière, APHP, and INSERM U1138 Paris, France.
Université de Paris, 75006 Paris, France.

Nhan Pham-Thi (N)

Unité de Neurophysiologie du Stress, Département des Neurosciences, Institut de Recherche Biomédicale des Armées (IRBA), BP 73 91223 Brétigny sur Orge Cedex, France.

Christiane Verny (C)

Unité de Neurophysiologie du Stress, Département des Neurosciences, Institut de Recherche Biomédicale des Armées (IRBA), BP 73 91223 Brétigny sur Orge Cedex, France.

Bruno Pitard (B)

Nantes Université, INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy, INCIT,UMR 1302, F-44000 Nantes, France.

Cyril Planchais (C)

Institut Pasteur, Université de Paris, Humoral Immunology Unit, Department of Immunology, 75015 Paris, France.

Hugo Mouquet (H)

Institut Pasteur, Université de Paris, Humoral Immunology Unit, Department of Immunology, 75015 Paris, France.

Richard Paul (R)

Institut Pasteur, Université de Paris, Functional Genetics of Infectious Diseases Unit, Department of Global Health, 75015 Paris, France.
Centre National de la Recherche Scientifique (CNRS), UMR2000, Paris Cedex 15, France.

Etienne Simon-Loriere (E)

Institut Pasteur, Université de Paris, G5 Evolutionary Genomics of RNA viruses, 75015 Paris, France.

Jagadeesh Bayry (J)

Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, 75006, France.
Department of Biological Sciences & Engineering, Indian Institute of Technology Palakkad, Palakkad 678623, India.

Laurent Gilardin (L)

Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, 75006, France.
Sorbonne Paris-Nord University (Paris 13), 93000 Bobigny, France.
Service de médecine interne, Hôpital Jean Verdier, HUPSSD, AP-HP, 93140 Bondy, France.

Anavaj Sakuntabhai (A)

Institut Pasteur, Université de Paris, Functional Genetics of Infectious Diseases Unit, Department of Global Health, 75015 Paris, France.
Centre National de la Recherche Scientifique (CNRS), UMR2000, Paris Cedex 15, France.
International Vaccine Design Center (vDesC), The Institute of Medical Science, The University of Tokyo (IMSUT), Tokyo, Japan.

Classifications MeSH