Tubular Mitochondrial Pyruvate Carrier Disruption Elicits Redox Adaptations that Protect from Acute Kidney Injury.

acute kidney injury metabolomics mitochondrial metabolism oxidative damage

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
31 Jan 2023
Historique:
pubmed: 14 2 2023
medline: 14 2 2023
entrez: 13 2 2023
Statut: epublish

Résumé

Energy-intensive kidney reabsorption processes essential for normal whole-body function are maintained by tubular epithelial cell metabolism. Tubular metabolism changes markedly following acute kidney injury (AKI), but which changes are adaptive versus maladaptive remain poorly understood. In publicly available data sets, we noticed a consistent downregulation of the mitochondrial pyruvate carrier (MPC) after AKI, which we experimentally confirmed. To test the functional consequences of MPC downregulation, we generated novel tubular epithelial cell-specific

Identifiants

pubmed: 36778297
doi: 10.1101/2023.01.31.526492
pmc: PMC9915694
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK104998
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA086862
Pays : United States
Organisme : NICHD NIH HHS
ID : K12 HD027748
Pays : United States
Organisme : NIAMS NIH HHS
ID : R00 AR070914
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007690
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK126991
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA217797
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES005605
Pays : United States

Déclaration de conflit d'intérêts

COMPETING INTERESTS The authors declare no competing interests.

Auteurs

Adam J Rauckhorst (AJ)

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.
Fraternal Order of Eagles Diabetes Research Center (FOEDRC), University of Iowa, Iowa City, IA, USA.
FOEDRC Metabolomics Core Research Facility, University of Iowa, Iowa City, IA, USA.

Gabriela Vasquez Martinez (GV)

Kidney and Urinary Tract Research Center, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus OH, USA.

Gabriel Mayoral Andrade (GM)

Kidney and Urinary Tract Research Center, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus OH, USA.

Hsiang Wen (H)

Stead Family Department of Pediatrics, University of Iowa, Iowa City, IA, USA.

Ji Young Kim (JY)

Division of Pharmaceutics and Pharmacology, College of Pharmacy & Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.

Aaron Simoni (A)

Kidney and Urinary Tract Research Center, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus OH, USA.

Kranti A Mapuskar (KA)

Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.

Prerna Rastogi (P)

Department of Pathology, University of Iowa, Iowa City, IA, USA.

Emily J Steinbach (EJ)

Stead Family Department of Pediatrics, University of Iowa, Iowa City, IA, USA.
Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.

Michael L McCormick (ML)

Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.

Bryan G Allen (BG)

Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.

Navjot S Pabla (NS)

Division of Pharmaceutics and Pharmacology, College of Pharmacy & Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.

Ashley R Jackson (AR)

Kidney and Urinary Tract Research Center, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus OH, USA.
Department of Pediatrics, The Ohio State University, Columbus, OH, USA.

Mitchell C Coleman (MC)

Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.
Department of Orthopedics and Rehabilitation, University of Iowa, Iowa City, IA, USA.

Douglas R Spitz (DR)

Free Radical and Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.

Eric B Taylor (EB)

Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA.
Fraternal Order of Eagles Diabetes Research Center (FOEDRC), University of Iowa, Iowa City, IA, USA.
FOEDRC Metabolomics Core Research Facility, University of Iowa, Iowa City, IA, USA.
Holden Comprehensive Cancer Center, University of Iowa, Iowa City, IA, USA.
Pappajohn Biomedical Institute, University of Iowa, Iowa City, IA, USA.

Diana Zepeda-Orozco (D)

Kidney and Urinary Tract Research Center, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus OH, USA.
Stead Family Department of Pediatrics, University of Iowa, Iowa City, IA, USA.
Department of Pediatrics, The Ohio State University, Columbus, OH, USA.

Classifications MeSH