Common laboratory diets differentially influence zebrafish gut microbiome's successional development and sensitivity to pathogen exposure.

Development Diet Gut microbiome Husbandry Infection Mycobacterium chelonae Zebrafish

Journal

Research square
Titre abrégé: Res Sq
Pays: United States
ID NLM: 101768035

Informations de publication

Date de publication:
02 Feb 2023
Historique:
pubmed: 14 2 2023
medline: 14 2 2023
entrez: 13 2 2023
Statut: epublish

Résumé

Despite the long-established importance of zebrafish Our analysis finds that diet has a substantial impact on the composition of the gut microbiome in adult fish, and that diet also impacts the developmental variation in the gut microbiome. We further evaluated whether the 7-month-old fish microbiome compositions that result from dietary variation are differentially sensitive to infection by a common laboratory pathogen, Overall, our results indicate that diet drives the successional development of the gut microbiome as well as its sensitivity to exogenous exposure. Consequently, investigators should carefully consider the role of diet in their microbiome zebrafish investigations, especially when integrating results across studies that vary by diet.

Sections du résumé

Background UNASSIGNED
Despite the long-established importance of zebrafish
Results UNASSIGNED
Our analysis finds that diet has a substantial impact on the composition of the gut microbiome in adult fish, and that diet also impacts the developmental variation in the gut microbiome. We further evaluated whether the 7-month-old fish microbiome compositions that result from dietary variation are differentially sensitive to infection by a common laboratory pathogen,
Conclusions UNASSIGNED
Overall, our results indicate that diet drives the successional development of the gut microbiome as well as its sensitivity to exogenous exposure. Consequently, investigators should carefully consider the role of diet in their microbiome zebrafish investigations, especially when integrating results across studies that vary by diet.

Identifiants

pubmed: 36778316
doi: 10.21203/rs.3.rs-2530939/v1
pmc: PMC9915791
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIH HHS
ID : P40 OD011021
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES030226
Pays : United States
Organisme : NIH HHS
ID : R24 OD010998
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing Interests The authors declare that they have no competing interests.

Auteurs

Michael Sieler (M)

Oregon State University.

Colleen Al-Samarrie (C)

Oregon State University.

Kristin Kasschau (K)

Oregon State University.

Zoltan Varga (Z)

University of Oregon.

Michael Kent (M)

Oregon State University.

Thomas Sharpton (T)

Oregon State University.

Classifications MeSH