Advantages of aggregation-induced luminescence microspheres compared with fluorescent microspheres in immunochromatography assay with sandwich format.

Luminescence Microspheres Reproducibility of Results Coloring Agents Chromatography, Affinity / methods Fluoresceins Immunoassay / methods
Aggregation-induced luminescence microspheres Detection performance Immunochromatography assay Organic fluorescein dye-embedded microspheres Procalcitonin

Journal

Analytica chimica acta
ISSN: 1873-4324
Titre abrégé: Anal Chim Acta
Pays: Netherlands
ID NLM: 0370534

Informations de publication

Date de publication:
22 Mar 2023
Historique:
received: 15 12 2022
accepted: 20 01 2023
entrez: 13 2 2023
pubmed: 14 2 2023
medline: 16 2 2023
Statut: ppublish

Résumé

Organic fluorescein dye-embedded fluorescent microspheres (FMs) are currently the most established commercially fluorescent markers, and they have been widely used to improve the sensitivity of immunochromatography assay (ICA). However, these FMs have natural defects, such as the aggregation-caused quenching effect and small Stokes shift, which are not conducive to improving the detection performance of ICA. Herein, two green emitted FMs, namely aggregation-induced emission FMs (AIEFMs) and fluorescein isothiocyanate FMs (FITCFMs), were prepared by swelling the AIE luminogens and FITC dyes into the carboxyl group-modified polystyrene microspheres. The average diameters of AIEFMs and FITCFMs were 350 and 450 nm, respectively. Compared with FITCFMs, the AIEFMs exhibited stronger fluorescence intensity and a larger Stokes shift. These two FMs were used as the labeling markers of ICA for procalcitonin (PCT) detection with the sandwich format. Among them, AIEFM-ICA showed dynamic linear detection of PCT from 7.6 pg mL

Identifiants

DOI: 10.1016/j.aca.2023.340869 PMID: 36781245
pubmed: 36781245
pii: S0003-2670(23)00090-9
doi: 10.1016/j.aca.2023.340869
pii:
doi:

Substances chimiques

Coloring Agents 0
Fluoresceins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

340869

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Xiaxia Wei (X)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Xirui Chen (X)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Zilong Liu (Z)

School of Food Science and Engineering, Hainan University, Haikou, 570228, China.

Ge Xu (G)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Guoxin Chen (G)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Xiangkai Lin (X)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Wenchao Zhan (W)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China.

Xiaolin Huang (X)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China. Electronic address: hxl19880503@163.com.

Yonghua Xiong (Y)

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China; School of Food Science and Technology, Nanchang University, Nanchang, 330031, China; Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, 330047, China. Electronic address: yhxiongchen@163.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiques › Rayonnement › Rayonnement non ionisant › Lumière › Luminescence › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Équipement et fournitures › Microsphères › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents colorants › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'affinité › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Composés polycycliques › Spiranes › Fluorescéines › Advantages of aggregation-induced luminescence...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Advantages of aggregation-induced luminescence...