Ultralow Limit Detection of Soluble HER2 Biomarker in Serum with a Fiber-Optic Ball-Tip Resonator Assisted by a Tilted FBG.
Journal
ACS measurement science au
ISSN: 2694-250X
Titre abrégé: ACS Meas Sci Au
Pays: United States
ID NLM: 9918232604306676
Informations de publication
Date de publication:
17 Aug 2022
17 Aug 2022
Historique:
entrez:
14
2
2023
pubmed:
15
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
An optical-fiber biosensor has been developed for the detection of the breast cancer biomarker soluble human epidermal growth factor receptor-2 (sHER2). The sensor was fabricated by combining a tilted fiber Bragg grating (TFBG) with a ball resonator, allowing us to achieve an excellent sensitivity compared to other optical-fiber-based sensors. The sensor exhibits a resonance comb excited by the TFBG and the spectral profile of the ball resonator. The detection of sHER2 at extremely low concentrations was carried out by tracking the amplitude change of selected resonances. The therapeutic anti-HER2 monoclonal antibody Trastuzumab has been used to functionalize the biosensor with silane surface chemistry. The sensor features a sensitivity of 4034 dB/RIU with a limit of detection (LoD) in buffer and in a 1/10 diluted serum of 151.5 ag/mL and 3.7 pg/mL, respectively. At relatively high protein concentrations (64 ng/mL) binding to sHER (7.36 dB) as compared to control proteins (below 0.7 dB) attested the high specificity of sHER2 detection.
Identifiants
pubmed: 36785571
doi: 10.1021/acsmeasuresciau.2c00008
pmc: PMC9885947
doi:
Types de publication
Journal Article
Langues
eng
Pagination
309-316Informations de copyright
© 2022 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Opt Express. 2020 Mar 2;28(5):7539-7551
pubmed: 32225979
Biosens Bioelectron. 2019 Apr 15;131:104-112
pubmed: 30826644
Biosens Bioelectron. 2017 Jun 15;92:449-456
pubmed: 27839732
Biosensors (Basel). 2017 Jun 21;7(2):
pubmed: 28635665
Opt Express. 2018 Sep 3;26(18):24154-24163
pubmed: 30184907
Sci Rep. 2021 Oct 1;11(1):19583
pubmed: 34599251
Anal Chem. 2009 Nov 15;81(22):9499-506
pubmed: 19848413
J Oncol. 2012;2012:413256
pubmed: 22570650
Rev Sci Instrum. 2007 Apr;78(4):046106
pubmed: 17477697
Am J Clin Pathol. 1994 Apr;101(4):465-70
pubmed: 8160638
Sensors (Basel). 2017 Nov 06;17(11):
pubmed: 29113127
Int J Mol Sci. 2017 Aug 25;18(9):
pubmed: 28841163
Oncologist. 2003;8(4):307-25
pubmed: 12897328
Sensors (Basel). 2017 Oct 17;17(10):
pubmed: 29039804
Sci Transl Med. 2012 Mar 28;4(127):127rv2
pubmed: 22461643
J Zhejiang Univ Sci B. 2008 Jan;9(1):5-9
pubmed: 18196606
Talanta. 2021 Jan 1;221:121452
pubmed: 33076075
Sensors (Basel). 2020 Oct 30;20(21):
pubmed: 33143287
Clin Cancer Res. 2000 Jun;6(6):2363-70
pubmed: 10873088
Cancer. 2011 May 1;117(9):1837-46
pubmed: 21509760
Biosens Bioelectron. 2017 May 15;91:15-23
pubmed: 27984706
Biomed Opt Express. 2020 Aug 04;11(9):4862-4871
pubmed: 33014586
Lab Invest. 2018 Jun;98(6):696-707
pubmed: 29491426
Biosens Bioelectron. 2018 May 1;104:158-162
pubmed: 29331430
Biosens Bioelectron. 2019 Dec 15;146:111765
pubmed: 31606689