The first taxonomic and functional characterization of human CAVD-associated microbiota.
T cells
aortic valve disease
immune response
microbiota
valve calcification
Journal
Microbial cell (Graz, Austria)
ISSN: 2311-2638
Titre abrégé: Microb Cell
Pays: Austria
ID NLM: 101632887
Informations de publication
Date de publication:
06 Feb 2023
06 Feb 2023
Historique:
received:
04
11
2022
revised:
23
12
2022
accepted:
09
01
2023
entrez:
15
2
2023
pubmed:
16
2
2023
medline:
16
2
2023
Statut:
epublish
Résumé
Calcific aortic valve disease (CAVD) is the most common heart valve disorder, defined by a remodeling multistep process: namely, valve fibrosis with its area narrowing, impaired blood flow, and final calcification phase. Nowadays, the only treatment is the surgical valve replacement. As for other cardiovascular diseases, growing evidence suggest an active role of the immune system in the calcification process that could be modulated by the microbiota. To address this point, we aimed to investigate and characterize, for the first time, the presence of a valve microbiota and associated immune response in human CAVD. Calcified aortic valve (CAV) samples from twenty patients (11 from Germany and 9 from Italy) with diagnosis of severe symptomatic CAVD were used to assess the presence of infiltrating T cells, by cloning approach, and to characterize the valve microbiota, by 16S rRNA gene sequencing (NGS). We documented the presence of infiltrating T lymphocytes, especially the T helper subset, in CAV samples. Moreover, we found a tissue-associated microbiota in freshly collected CAV samples, which was significantly different in Italian and German patients, suggesting potential correlation with other cardiovascular risk factors. The presence of microbiota in inflamed CAV samples represents the right trigger point to explain the valve calcification process, encouraging further studies to explore the potential link between bacteria and adaptive immune response and to define the critical role of local microbiota-immunity axis on CAVD development.
Identifiants
pubmed: 36789351
doi: 10.15698/mic2023.02.791
pii: MIC0272E138
pmc: PMC9896411
doi:
Types de publication
Journal Article
Langues
eng
Pagination
36-48Informations de copyright
Copyright: © 2023 Curini et al.
Déclaration de conflit d'intérêts
Conflict of Interest: The authors have declared no conflict of interest.
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