Imaging Voltage Globally and in Isofrequency Lamina in Slices of Mouse Ventral Cochlear Nucleus.
auditory nerve
c-fos
cochlear nuclei
genetically-encoded voltage sensors
tonotopic organization
voltage imaging
Journal
eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
15
11
2022
revised:
02
02
2023
accepted:
06
02
2023
pubmed:
16
2
2023
medline:
10
3
2023
entrez:
15
2
2023
Statut:
epublish
Résumé
The cochlear nuclei (CNs) receive sensory information from the ear and perform fundamental computations before relaying this information to higher processing centers. These computations are performed by distinct types of neurons interconnected in circuits dedicated to the specialized roles of the auditory system. In the present study, we explored the use of voltage imaging to investigate CN circuitry. We tested two approaches based on fundamentally different voltage sensing technologies. Using a voltage-sensitive dye we recorded glutamate receptor-independent signals arising predominantly from axons. The mean conduction velocity of these fibers of 0.27 m/s was rapid but in range with other unmyelinated axons. We then used a genetically-encoded hybrid voltage sensor (hVOS) to image voltage from a specific population of neurons. Probe expression was controlled using Cre recombinase linked to
Identifiants
pubmed: 36792362
pii: ENEURO.0465-22.2023
doi: 10.1523/ENEURO.0465-22.2023
pmc: PMC9997695
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS093866
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS127219
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC016861
Pays : United States
Organisme : NIDCD NIH HHS
ID : R21 DC014858
Pays : United States
Informations de copyright
Copyright © 2023 Ma et al.
Déclaration de conflit d'intérêts
The authors declare no competing financial interests.
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