Atlas-scale single-cell chromatin accessibility using nanowell-based combinatorial indexing.


Journal

Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021

Informations de publication

Date de publication:
02 2023
Historique:
received: 31 01 2022
accepted: 08 12 2022
pubmed: 16 2 2023
medline: 8 3 2023
entrez: 15 2 2023
Statut: ppublish

Résumé

Here we present advancements in single-cell combinatorial indexed Assay for Transposase Accessible Chromatin (sciATAC) to measure chromatin accessibility that leverage nanowell chips to achieve atlas-scale cell throughput (>10

Identifiants

pubmed: 36792372
pii: gr.276655.122
doi: 10.1101/gr.276655.122
pmc: PMC10069466
doi:

Substances chimiques

Chromatin 0
Transposases EC 2.7.7.-

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

208-217

Subventions

Organisme : NIMH NIH HHS
ID : RF1 MH128842
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA047237
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM124704
Pays : United States

Informations de copyright

© 2023 O'Connell et al.; Published by Cold Spring Harbor Laboratory Press.

Références

Nat Commun. 2021 Feb 24;12(1):1274
pubmed: 33627658
Nat Genet. 2021 Mar;53(3):403-411
pubmed: 33633365
Nat Commun. 2018 Sep 7;9(1):3647
pubmed: 30194434
Nat Neurosci. 2018 Mar;21(3):432-439
pubmed: 29434377
Cell. 2018 Aug 9;174(4):999-1014.e22
pubmed: 30096314
Nat Commun. 2018 Dec 17;9(1):5345
pubmed: 30559361
Neuron. 2005 Jan 20;45(2):207-21
pubmed: 15664173
Nature. 2020 Jul;583(7818):699-710
pubmed: 32728249
Science. 2015 May 22;348(6237):910-4
pubmed: 25953818
Genome Biol. 2008;9(9):R137
pubmed: 18798982
Front Cell Neurosci. 2020 Feb 19;14:31
pubmed: 32140099
Nat Biotechnol. 2019 Aug;37(8):916-924
pubmed: 31235917
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Nat Methods. 2019 May;16(5):397-400
pubmed: 30962623
Cell. 2021 Jun 10;184(12):3222-3241.e26
pubmed: 34004146
Nat Methods. 2019 Dec;16(12):1289-1296
pubmed: 31740819
Nat Biotechnol. 2019 Aug;37(8):925-936
pubmed: 31375813
Nat Commun. 2019 Feb 4;10(1):581
pubmed: 30718509
Science. 2020 Nov 13;370(6518):
pubmed: 33184180
Genome Res. 2019 May;29(5):857-869
pubmed: 30936163
Cell. 2015 Jul 2;162(1):184-97
pubmed: 26095251
Nat Methods. 2013 Dec;10(12):1213-8
pubmed: 24097267
Nat Biotechnol. 2021 Dec;39(12):1574-1580
pubmed: 34226710

Auteurs

Brendan L O'Connell (BL)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Ruth V Nichols (RV)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Dmitry Pokholok (D)

ScaleBio, San Diego, California 92121, USA.

Jerushah Thomas (J)

ScaleBio, San Diego, California 92121, USA.

Sonia N Acharya (SN)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Andrew Nishida (A)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Casey A Thornton (CA)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Marissa Co (M)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Andrew J Fields (AJ)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA.

Frank J Steemers (FJ)

ScaleBio, San Diego, California 92121, USA.

Andrew C Adey (AC)

Oregon Health & Science University, Department of Molecular and Medical Genetics, Portland, Oregon 97239, USA; adey@ohsu.edu.
Oregon Health & Science University, Knight Cancer Institute, Portland, Oregon 97239, USA.
Oregon Health & Science University, Cancer Early Detection Advanced Research Center, Portland, Oregon 97239, USA.
Oregon Health & Science University, Knight Cardiovascular Institute, Portland, Oregon 97239, USA.

Articles similaires

Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH