Uphill energy transfer mechanism for photosynthesis in an Antarctic alga.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
15 02 2023
Historique:
received: 27 07 2021
accepted: 20 01 2023
entrez: 15 2 2023
pubmed: 16 2 2023
medline: 18 2 2023
Statut: epublish

Résumé

Prasiola crispa, an aerial green alga, forms layered colonies under the severe terrestrial conditions of Antarctica. Since only far-red light is available at a deep layer of the colony, P. crispa has evolved a molecular system for photosystem II (PSII) excitation using far-red light with uphill energy transfer. However, the molecular basis underlying this system remains elusive. Here, we purified a light-harvesting chlorophyll (Chl)-binding protein complex from P. crispa (Pc-frLHC) that excites PSII with far-red light and revealed its ring-shaped structure with undecameric 11-fold symmetry at 3.13 Å resolution. The primary structure suggests that Pc-frLHC evolved from LHCI rather than LHCII. The circular arrangement of the Pc-frLHC subunits is unique among eukaryote LHCs and forms unprecedented Chl pentamers at every subunit‒subunit interface near the excitation energy exit sites. The Chl pentamers probably contribute to far-red light absorption. Pc-frLHC's unique Chl arrangement likely promotes PSII excitation with entropy-driven uphill excitation energy transfer.

Identifiants

pubmed: 36792917
doi: 10.1038/s41467-023-36245-1
pii: 10.1038/s41467-023-36245-1
pmc: PMC9931709
doi:

Substances chimiques

Photosystem I Protein Complex 0
Photosystem II Protein Complex 0
Light-Harvesting Protein Complexes 0
Chlorophyll 1406-65-1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

730

Informations de copyright

© 2023. The Author(s).

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Auteurs

Makiko Kosugi (M)

Astrobiology Center, 2-21-1 Osawa, Mitaka, Tokyo, 181-8588, Japan. mkosugi@nibb.ac.jp.
National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo, 181-8588, Japan. mkosugi@nibb.ac.jp.
Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan. mkosugi@nibb.ac.jp.
National Institute for Basic Biology, National Institutes of Natural Science, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan. mkosugi@nibb.ac.jp.

Masato Kawasaki (M)

Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.
Department of Materials Structure Science, School of High Energy Accelerator Science, The Graduate University of Advanced Studies (Soken-dai), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.

Yutaka Shibata (Y)

Department of Chemistry, Graduate School of Science, Tohoku University, 6-3 Aza Aoba Aramaki, Aoba-ku, Sendai, 980-8578, Japan. shibata@m.tohoku.ac.jp.

Kojiro Hara (K)

Department of Biological Production, Akita Prefectural University, 241-438 Kaidobata-nishi, Shimoshinjo-nakano, Akita, 010-0195, Japan.

Shinichi Takaichi (S)

Department of Molecular Microbiology, Tokyo University of Agriculture, 1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan.

Toshio Moriya (T)

Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.

Naruhiko Adachi (N)

Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan.

Yasuhiro Kamei (Y)

National Institute for Basic Biology, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan.
Department of Basic Biology, School of Life Sciences, SOKENDAI (The Graduate University for Advanced Studies), 38 Nishigonaka, Myodaiji, Okazaki, Aichi, 444-8585, Japan.

Yasuhiro Kashino (Y)

Graduate School of Science, University of Hyogo, 3-2-1 Kohto, Kamigohri, Ako-gun, Hyogo, 678-1297, Japan.

Sakae Kudoh (S)

National Institute of Polar Research, Research Organization of Information and Systems, 10-3 Midori-cho, Tachikawa, Tokyo, 190-8518, Japan.
Department of Polar Science, School of Multidisciplinary Science, SOKENDAI (The Graduate University for Advanced Studies), 10-3 Midori-cho, Tachikawa, Tokyo, 190-8518, Japan.

Hiroyuki Koike (H)

Department of Biological Sciences, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan.

Toshiya Senda (T)

Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan. toshiya.senda@kek.jp.
Department of Materials Structure Science, School of High Energy Accelerator Science, The Graduate University of Advanced Studies (Soken-dai), 1-1 Oho, Tsukuba, Ibaraki, 305-0801, Japan. toshiya.senda@kek.jp.
Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. toshiya.senda@kek.jp.

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Classifications MeSH