Comprehensive insights into the metabolism characteristics of small RNA Qrr4 in Vibrio alginolyticus.
Lipidomics
Metabolomics
Small noncoding RNAs
Vibrio alginolyticus
Virulence
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
23
11
2022
accepted:
31
01
2023
revised:
30
01
2023
pubmed:
17
2
2023
medline:
15
3
2023
entrez:
16
2
2023
Statut:
ppublish
Résumé
Vibrio alginolyticus is an important foodborne pathogen that can infect both humans and marine animals and cause massive economic losses in aquaculture. Small noncoding RNAs (sRNAs) are emerging posttranscriptional regulators that affect bacterial physiology and pathological processes. In the present work, a new cell density-dependent sRNA, Qrr4, was characterized in V. alginolyticus based on a previously reported RNA-seq analysis and bioinformatics approach. The effects of Qrr4 actions on the physiology, virulence, and metabolism of V. alginolyticus were comprehensively investigated based on molecular biology and metabolomics approaches. The results showed that qrr4 deletion markedly inhibited growth, motility and extracellular protease activities. Additionally, nontargeted metabolism and lipidomics analyses revealed that qrr4 deletion induced significant disturbance of multiple metabolic pathways. The key metabolic remodelling that occurred in response to qrr4 deletion was found to involve phospholipid, nucleotide, carbohydrate and amino acid metabolic pathways, providing novel clues about a potential mechanism via which mutation of qrr4 could interfere with cellular energy homeostasis, modulate membrane phospholipid composition and inhibit nucleic acid and protein syntheses to regulate the motility, growth and virulence characteristics of V. alginolyticus. Overall, this study provides a comprehensive understanding of the regulatory roles of the new cell density-dependent sRNA Qrr4 in V. alginolyticus. KEY POINTS: • A novel cell density-dependent sRNA, Qrr4, was cloned in V. alginolyticus. •Qrr4 regulated growth and virulence factors of V. alginolyticus. • Phospholipid, nucleotide and energy metabolisms were modulated obviously by Qrr4.
Identifiants
pubmed: 36795140
doi: 10.1007/s00253-023-12435-1
pii: 10.1007/s00253-023-12435-1
doi:
Substances chimiques
Virulence Factors
0
Nucleotides
0
RNA, Small Untranslated
0
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1887-1902Subventions
Organisme : National Natural Science Foundation of China
ID : 31800328
Organisme : National Natural Science Foundation of China
ID : 32070129
Organisme : Key Research Project of Shaanxi Provincial Science and Technology Department
ID : 2021NY-164
Organisme : Key Research Project of Shaanxi Provincial Science and Technology Department
ID : 2023-YBNY-171
Organisme : Young Talent Fund of University Association for Science and Technology of Shaanxi
ID : No.20210213
Organisme : Xi'an Science and Technology Project
ID : 22NYYF036
Organisme : Hebei Province Natural Science Foundation
ID : B2019415024
Organisme : Hebei Province Scientific Research Key Projects of Education Department
ID : ZD2021038
Organisme : Key Research and Development Program of Hebei Province under Grant
ID : 20373902D
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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