Antibody Immobilization.

Antibodies / chemistry Enzyme-Linked Immunosorbent Assay Biotin Streptavidin

Antibody Biotin Chemistry Coupling Density Immobilization Oriented Passive Protein A Random Solid phase Steric hindrance Streptavidin

Journal

Methods in molecular biology (Clifton, N.J.)

ISSN: 1940-6029

Titre abrégé: Methods Mol Biol

Pays: United States

ID NLM: 9214969

Informations de publication

Date de publication:
2023

Historique:

entrez: 16 2 2023

pubmed: 17 2 2023

medline: 22 2 2023

Statut: ppublish

Résumé

In the ELISA format(s), the capture antibody is usually affixed to a solid phase, commonly referred to as the immunosorbent. How to tether the antibody most effectively will depend upon the physical properties of the support (plate well, latex bead, flow cell, etc.) as well as its chemical nature (hydrophobic, hydrophilic, the presence of reactive groups such as epoxide, etc.). Of course, it is ultimately the suitability of the antibody to withstand the linking process while preserving its antigen-binding efficiency that must be determined. In this chapter, the antibody immobilization processes and their consequences are described.

Identifiants

DOI: 10.1007/978-1-0716-2903-1_3 PMID: 36795357

pubmed: 36795357

doi: 10.1007/978-1-0716-2903-1_3

doi:

Substances chimiques

Antibodies 0

Biotin 6SO6U10H04

Streptavidin 9013-20-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

33-44

Informations de copyright

Références

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Antibody Immobilization.

Journal

Informations de publication

Résumé

Identifiants

Substances chimiques

Types de publication

Langues

Sous-ensembles de citation

Pagination

Informations de copyright

Références

Auteurs

Robert S Matson (RS)

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