Well-Based Multiplex Food Allergen Colorimetric ELISA.

Allergens Allergy Colorimetric ELISA FDA HRP IgE IgG IgG4 Immunoassay Intolerance Microarray Microplate Multiplex Plasma Protein extracts Strip-well

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 16 2 2023
pubmed: 17 2 2023
medline: 22 2 2023
Statut: ppublish

Résumé

Food allergy is a well-recognized and significant health hazard around the world. At least 160 food groups have been identified that present allergenic reactions or other sensitivities and intolerance in humans. Enzyme-linked immunosorbent assay (ELISA) is an accepted platform for identifying the nature of the food allergy and its severity. It is now possible to simultaneously screen patients for allergic sensitivity and intolerance to multiple allergens using multiplex immunoassays. This chapter describes the preparation and utility of a multiplex allergen ELISA for the assessment of food allergy and sensitivity in patients.

Identifiants

pubmed: 36795367
doi: 10.1007/978-1-0716-2903-1_13
doi:

Substances chimiques

Immunosorbents 0
Immunoglobulin E 37341-29-0
Allergens 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

169-181

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

https://www.fda.gov/food/food-labeling-nutrition/food-allergies
https://www.fda.gov/food/buy-store-serve-safe-food/food-allergies-what-you-need-know
David NA, Penumarti A, Burks AW et al (2017) Food allergen extracts to diagnose food-induced allergic diseases: how they are made. Ann Allergy Asthma Immunol 119:101–107
doi: 10.1016/j.anai.2016.11.008 pubmed: 28801015
Wiltshire S, O’Malley S, Lambert J, Kukanskis K, Edgar D et al (2000) Detection of multiple allergen-specific IgEs on microarrays by immunoassay with rolling circle amplification. Clin Chem 46(12):1990–1993
doi: 10.1093/clinchem/46.12.1990 pubmed: 11106333
Lebrun SJ, Petchpuda WN, Huia A, McLaughlin CS (2005) Development of a sensitive, colorometric microarray assay for allergen responsive human IgE. J Immunol Methods 300:24–31
doi: 10.1016/j.jim.2005.01.019 pubmed: 15893759
Vigh-Conrad KA, Conrad DF, Preuss D (2010) A protein allergen microarray detects specific IgE to pollen surface, cytoplasmic, and commercial allergen extracts. PLoS One 5(4):e10174. https://doi.org/10.1371/journal.pone.0010174
doi: 10.1371/journal.pone.0010174 pubmed: 20419087 pmcid: 2856625
Feyzkhanova G, Voloshin S, Smoldovskaya O, Arefieva A et al (2017) Development of a microarray-based method for allergen-specific IgE and IgG4 detection. Clin Proteomics 14(1):1–13. https://doi.org/10.1186/s12014-016-9136-7
doi: 10.1186/s12014-016-9136-7 pubmed: 28077935 pmcid: 5223422
Matson RS (2016) Demonstration of a multiplex milk allergen ELISA using oligonucleotide-tethered principal component proteins. SOJ Biochem 2(1):1–7
doi: 10.15226/2376-4589/2/1/00109

Auteurs

Robert S Matson (RS)

QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

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