Super-resolution SRS microscopy with A-PoD.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
11
10
2021
accepted:
17
01
2023
pubmed:
17
2
2023
medline:
14
3
2023
entrez:
16
2
2023
Statut:
ppublish
Résumé
Stimulated Raman scattering (SRS) offers the ability to image metabolic dynamics with high signal-to-noise ratio. However, its spatial resolution is limited by the numerical aperture of the imaging objective and the scattering cross-section of molecules. To achieve super-resolved SRS imaging, we developed a deconvolution algorithm, adaptive moment estimation (Adam) optimization-based pointillism deconvolution (A-PoD) and demonstrated a spatial resolution of lower than 59 nm on the membrane of a single lipid droplet (LD). We applied A-PoD to spatially correlated multiphoton fluorescence imaging and deuterium oxide (D
Identifiants
pubmed: 36797410
doi: 10.1038/s41592-023-01779-1
pii: 10.1038/s41592-023-01779-1
pmc: PMC10246886
mid: NIHMS1896464
doi:
Substances chimiques
Proteins
0
Lipids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
448-458Subventions
Organisme : NCI NIH HHS
ID : U54 CA137788
Pays : United States
Organisme : NHLBI NIH HHS
ID : U54 HL165443
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS125395
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA132378
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK134301
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS111039
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.
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