Mitochondrial genome sequencing and analysis of the invasive

Poaceae grass invasion genomics long read sequencing mitogenome

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
11 Feb 2023
Historique:
pubmed: 18 2 2023
medline: 18 2 2023
entrez: 17 2 2023
Statut: epublish

Résumé

Plants remain underrepresented among species with sequenced mitochondrial genomes (mitogenomes), due to the difficulty in assembly with short-read technology. Invasive species lag behind crops and other economically important species in this respect, representing a lack of tools for management and land conservation efforts. The mitogenome of The assembly is 478,010 bp in length and characterized by two large, inverted repeats, and a large, direct repeat. However, the genome could not be circularized, arguing against a "master circle" structure. Long-read assemblies with data subsets revealed several alternative genomic conformations, predominantly associated with large repeats. Plastid-like sequences comprise 2.4% of the genome, with further evidence of Class I and Class II transposable element-like sequences. Phylogenetic analysis placed We present an in-depth analysis of mitogenome structure, content, phylogenetic relationships, and range-wide genomic variation in

Identifiants

pubmed: 36798355
doi: 10.1101/2023.02.10.527995
pmc: PMC9934601
pii:
doi:

Types de publication

Preprint

Langues

eng

Auteurs

Craig F Barrett (CF)

Department of Biology, West Virginia University, 53 Campus Drive, Morgantown, West Virginia, USA 26506.

Dhanushya Ramachandran (D)

Department of Biology, West Virginia University, 53 Campus Drive, Morgantown, West Virginia, USA 26506.

Chih-Hui Chen (CH)

Endemic Species Research Institute, 1 Ming-Sheng East Road, Jiji, Nantou 552, Taiwan.

Cameron W Corbett (CW)

Department of Biology, West Virginia University, 53 Campus Drive, Morgantown, West Virginia, USA 26506.

Cynthia D Huebner (CD)

Department of Biology, West Virginia University, 53 Campus Drive, Morgantown, West Virginia, USA 26506.
USDA Forest Service, Northern Research Station, 180 Canfield Street, Morgantown, West Virginia, USA 26505.
Division of Plant and Soil Sciences, West Virginia University, 204 Evansdale Greenhouse, Morgantown, West Virginia, USA 26506.

Brandon T Sinn (BT)

Department of Biology and Earth Science, Otterbein University, 1 South Grove Street, Westerville, OH USA 43081.
Faculty of Biology, University of Latvia, 1 Jelgavas iela, Riga, Latvia LV-1004.

Wen-Bin Yu (WB)

Center for Integrative Conservation Xishuangbanna Tropical Botanical Garden, CAS Mengla, Yunnan 666303, China.

Kenji Suetsugu (K)

Department of Biology, Graduate School of Science, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe, 657-8501, Japan.

Classifications MeSH