Comparative transcriptome analysis of non-germinated and germinated spores of Enterocytozoon hepatopenaei (EHP) in vitro.
Enterocytozoon hepatopenaei
Germination
Transcriptome
Journal
Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
23
12
2022
revised:
12
02
2023
accepted:
13
02
2023
pubmed:
23
2
2023
medline:
3
3
2023
entrez:
22
2
2023
Statut:
ppublish
Résumé
Enterocytozoon hepatopenaei (EHP), an obligate intracellular parasite classified as microsporidia, is an emerging pathogen with a significant impact on the global shrimp aquaculture industry. The understanding of how microsporidia germinate has been a key factor in exploring its infection process. However, the germination process of EHP was rarely reported. To gain insight into the germination process, we conducted a high-throughput sequencing analysis of purified EHP spores that had undergone in vitro germination treatment. This analysis revealed 137 differentially expressed genes, with 84 up-regulated and 53 down-regulated genes. While the functions of some of the genes remain unknown, this study provides important data on the transcriptomic changes before and after EHP germination, which can aid in further studies on the EHP infection mechanism.
Identifiants
pubmed: 36806462
pii: S0022-2011(23)00017-4
doi: 10.1016/j.jip.2023.107900
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107900Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.