Ubiquitin-Conjugating Enzyme OsUBC11 Affects the Development of Roots via Auxin Pathway.
Auxin
Auxin transport
Root development
Ubiquitin degradation signal
Ubiquitin-conjugating enzyme
Journal
Rice (New York, N.Y.)
ISSN: 1939-8425
Titre abrégé: Rice (N Y)
Pays: United States
ID NLM: 101503136
Informations de publication
Date de publication:
20 Feb 2023
20 Feb 2023
Historique:
received:
22
09
2022
accepted:
11
02
2023
entrez:
22
2
2023
pubmed:
23
2
2023
medline:
23
2
2023
Statut:
epublish
Résumé
Rice has 48 ubiquitin-conjugating enzymes, and the functions of most of these enzymes have not been elucidated. In the present study, a T-DNA insertional mutant named R164, which exhibited a significant decrease in the length of primary and lateral roots, was used as the experimental material to explore the potential function of OsUBC11. Analysis using the SEFA-PCR method showed that the T-DNA insertion was present in the promoter region of OsUBC11 gene, which encodes ubiquitin-conjugating enzyme (E2), and activates its expression. Biochemical experiments showed that OsUBC11 is a lysine-48-linked ubiquitin chain-forming conjugase. OsUBC11 overexpression lines showed the same root phenotypes. These results demonstrated that OsUBC11 was involved in root development. Further analyses showed that the IAA content of R164 mutant and OE3 line were significantly lower compared with wild-type Zhonghua11. Application of exogenous NAA restored the length of lateral and primary roots in R164 and OsUBC11 overexpression lines. Expression of the auxin synthesis regulating gene OsYUCCA4/6/7/9, the auxin transport gene OsAUX1, auxin/indole-3-acetic acid (Aux/IAA) family gene OsIAA31, auxin response factor OsARF16 and root regulator key genes, including OsWOX11, OsCRL1, OsCRL5 was significantly down-regulated in OsUBC11 overexpressing plants. Collectively, these results indicate that OsUBC11 modulates auxin signaling, ultimately affecting root development at the rice seedling stage.
Identifiants
pubmed: 36808375
doi: 10.1186/s12284-023-00626-3
pii: 10.1186/s12284-023-00626-3
pmc: PMC9941415
doi:
Types de publication
Journal Article
Langues
eng
Pagination
9Subventions
Organisme : he Strategic Priority Research Program of the Chinese Academy of Sciences
ID : XDA28100301
Organisme : he National Natural Science Foundation of China
ID : U20A2025
Organisme : Chinese Postdoctoral Science Foundation
ID : 2021M693157
Organisme : Natural Science Foundation of Heilongjiang Province
ID : JJ2021YX0477
Organisme : the Opening Foundation of Key Laboratory of Germplasm Enhancement, Physiology and Ecology of Food Crops in Cold Region, Ministry of Education, Northeast Agricultural University
ID : CXSTOP2021006
Organisme : Major research and development project of Inner Mongolia
ID : 2020ZD0023
Informations de copyright
© 2023. The Author(s).
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