Chronic Cerebral Hypoperfusion-Induced Disturbed Proteostasis of Mitochondria and MAM Is Reflected in the CSF of Rats by Proteomic Analysis.
BCCAO
CSF
Chronic cerebral hypoperfusion
MAM
Mitochondria
Proteomics
Journal
Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
04
10
2022
accepted:
04
01
2023
medline:
25
4
2023
pubmed:
23
2
2023
entrez:
22
2
2023
Statut:
ppublish
Résumé
Declining cerebral blood flow leads to chronic cerebral hypoperfusion which can induce neurodegenerative disorders, such as vascular dementia. The reduced energy supply of the brain impairs mitochondrial functions that could trigger further damaging cellular processes. We carried out stepwise bilateral common carotid occlusions on rats and investigated long-term mitochondrial, mitochondria-associated membrane (MAM), and cerebrospinal fluid (CSF) proteome changes. Samples were studied by gel-based and mass spectrometry-based proteomic analyses. We found 19, 35, and 12 significantly altered proteins in the mitochondria, MAM, and CSF, respectively. Most of the changed proteins were involved in protein turnover and import in all three sample types. We confirmed decreased levels of proteins involved in protein folding and amino acid catabolism, such as P4hb and Hibadh in the mitochondria by western blot. We detected reduced levels of several components of protein synthesis and degradation in the CSF as well as in the subcellular fractions, implying that hypoperfusion-induced altered protein turnover of brain tissue can be detected in the CSF by proteomic analysis.
Identifiants
pubmed: 36808604
doi: 10.1007/s12035-023-03215-z
pii: 10.1007/s12035-023-03215-z
pmc: PMC10122630
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3158-3174Subventions
Organisme : Nemzeti Kutatási, Fejlesztési és Innovaciós Alap
ID : ÚNKP-21-3
Organisme : National Research, Development and Innovation Office
ID : 2017-1.2.1-NKP-2017-00002
Organisme : National Research, Development and Innovation Office
ID : FIEK_16-1-2016-0005
Organisme : National Research, Development and Innovation Office
ID : NAP2022-I- 3/2022
Organisme : ELTE Proof of Concept
ID : 2019-1.2.1-Egyetemi-ÖKO-2019-0004
Organisme : Economic Development and Innovation Operative Programs
ID : GINOP-2.3.2-15-2016-00001
Organisme : Economic Development and Innovation Operative Programs
ID : GINOP-2.3.2-15-2016-00020
Organisme : HORIZON EUROPE Reforming and enhancing the European Research and Innovation system
ID : No. 739593
Informations de copyright
© 2023. The Author(s).
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