Phosphorylation of ATF2 promotes odontoblastic differentiation via intrinsic HAT activity.

Animals Mice Cell Differentiation / genetics Chromatin / genetics Extracellular Matrix Proteins / genetics Odontoblasts / metabolism Phosphorylation
ATF Cell differentiation Chromatin accessibility Dental papilla Mesenchymal stem cells Odontoblast Transcription factors

Journal

Journal of genetics and genomics = Yi chuan xue bao
ISSN: 1673-8527
Titre abrégé: J Genet Genomics
Pays: China
ID NLM: 101304616

Informations de publication

Date de publication:
07 2023
Historique:
received: 31 10 2022
revised: 15 01 2023
accepted: 05 02 2023
medline: 24 7 2023
pubmed: 23 2 2023
entrez: 22 2 2023
Statut: ppublish

Résumé

Mouse dental papilla cells (mDPCs) are cranial neural crest-derived dental mesenchymal cells that give rise to dentin-secreting odontoblasts after the bell stage during odontogenesis. The odontoblastic differentiation of mDPCs is spatiotemporally regulated by transcription factors (TFs). Our previous work reveals that chromatin accessibility was correlated with the occupation of the basic leucine zipper TF family during odontoblastic differentiation. However, the detailed mechanism by which TFs regulate the initiation of odontoblastic differentiation remains elusive. Here, we report that phosphorylation of ATF2 (p-ATF2) is particularly increased during odontoblastic differentiation in vivo and in vitro. ATAC-seq and p-ATF2 CUT&Tag experiments further demonstrate a high correlation between p-ATF2 localization and increased chromatin accessibility of regions near mineralization-related genes. Knockdown of Atf2 inhibits the odontoblastic differentiation of mDPCs, while overexpression of p-ATF2 promotes odontoblastic differentiation. ATAC-seq after overexpression of p-ATF2 reveals that p-ATF2 increases the chromatin accessibility of regions adjacent to genes associated with matrix mineralization. Furthermore, we find that p-ATF2 physically interacts with and promotes H2BK12 acetylation. Taken together, our findings reveal a mechanism that p-ATF2 promotes odontoblastic differentiation at initiation via remodeling chromatin accessibility and emphasize the role of the phosphoswitch model of TFs in cell fate transitions.

Identifiants

DOI: 10.1016/j.jgg.2023.02.005 PMID: 36809837
pubmed: 36809837
pii: S1673-8527(23)00044-9
doi: 10.1016/j.jgg.2023.02.005
pii:
doi:

Substances chimiques

Chromatin 0
Extracellular Matrix Proteins 0
Atf2 protein, mouse 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

497-510

Informations de copyright

Copyright © 2023 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare that they have no conflicts of interest regarding this work.

Auteurs

Huanyan Zuo (H)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Yao Xiao (Y)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Jiahao Han (J)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Yuxiu Lin (Y)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China; Department of Cariology and Endodontics, School of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Cheng Tian (C)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Shu Zhang (S)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Guohua Yuan (G)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China.

Huan Liu (H)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China; Department of Periodontology, School of Stomatology, Wuhan University, Wuhan, Hubei 430079, China; TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei 430079, China. Electronic address: liu.huan@whu.edu.cn.

Zhi Chen (Z)

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, Hubei 430079, China; Department of Cariology and Endodontics, School of Stomatology, Wuhan University, Wuhan, Hubei 430079, China. Electronic address: zhichen@whu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Odontoblastes › Phosphorylation of ATF2 promotes odontoblastic...
questionsmedicales.fr › Métabolisme › Phosphorylation › Phosphorylation of ATF2 promotes odontoblastic...