Non-Affinity Purification of Antibodies.
MEP
Protein A
activated carbon
agarose
antibody
mixed-mode
non-affinity
purification
salting-out
Journal
Antibodies (Basel, Switzerland)
ISSN: 2073-4468
Titre abrégé: Antibodies (Basel)
Pays: Switzerland
ID NLM: 101587489
Informations de publication
Date de publication:
13 Feb 2023
13 Feb 2023
Historique:
received:
12
01
2023
revised:
01
02
2023
accepted:
09
02
2023
entrez:
22
2
2023
pubmed:
23
2
2023
medline:
23
2
2023
Statut:
epublish
Résumé
Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.
Identifiants
pubmed: 36810520
pii: antib12010015
doi: 10.3390/antib12010015
pmc: PMC9944463
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
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