Time-dependent effects of cellulose and gelatin-based hemostats on cellular processes of wound healing.
cell migration
cell proliferation
cellulose
cytokine
exposure
fibroblast
gelatin
hemostats
wound healing
Journal
Archives of medical science : AMS
ISSN: 1734-1922
Titre abrégé: Arch Med Sci
Pays: Poland
ID NLM: 101258257
Informations de publication
Date de publication:
2023
2023
Historique:
received:
13
03
2019
accepted:
21
07
2019
entrez:
23
2
2023
pubmed:
4
2
2020
medline:
4
2
2020
Statut:
epublish
Résumé
Oxidized regenerated cellulose-based (ORC - TABOTAMP), oxidized non-regenerated cellulose-based (ONRC - RESORBA CELL), and gelatin-based (GELA - GELITA TUFT-IT) hemostats are commonly used in surgery. However, their impact on the wound healing process remains largely unexplored. We here assess time-dependent effects of exposure to these hemostats on fibroblast-related wound healing processes. Hemostats were applied to fibroblast cell cultures for 5-10 (short-), 30 and 60 min (intermediate-) and 24 h (long-term). Representative images of the hemostat degradation process were obtained, and the pH value was measured. Cell viability, apoptosis and migration were analyzed after the above exposure times at 3, 6 and 24 h follow-up. Protein levels for tumor necrosis factor α (TNF-α) and transforming-growth factor β (TGF-β) were assessed. ORC and ONRC reduced pH values during degradation, while GELA proved to be pH-neutral. Hemostat structural integrity was prolonged for GELA (vs. ORC and ONRC). TGF-β and TNF-α levels were reduced for ORC and ONRC (vs. GELA and control) ( Short-term exposure to ORC and ONRC impairs relevant wound healing-related processes in fibroblasts, and alters protein levels of key mediating cytokines. GELA does not show similar effects. We conclude that GELA may be preferred over ORC and ONRC over short-, intermediate- and long-term exposures. Future validation of the clinical relevance is warranted.
Identifiants
pubmed: 36817681
doi: 10.5114/aoms.2020.92830
pii: 110918
pmc: PMC9897096
doi:
Types de publication
Journal Article
Langues
eng
Pagination
194-202Informations de copyright
Copyright: © 2020 Termedia & Banach.
Déclaration de conflit d'intérêts
All authors who are affiliated with the Department of Vascular and Endovascular Surgery, Heinrich-Heine University Düsseldorf, Germany and Alexander Oberhuber participated in the “GELITA Medical Gelatin Hemostat Postmarket Registry” (Registry-ID: 2016116001).
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