A Microfluidic Device for Long-Term Maintenance of Organotypic Liver Cultures.
Hepatocytes
Liver
Microfluidics
Organotypic cultures
Organs-on-a-chip
Journal
Advanced materials technologies
ISSN: 2365-709X
Titre abrégé: Adv Mater Technol
Pays: United States
ID NLM: 101717732
Informations de publication
Date de publication:
24 Jan 2023
24 Jan 2023
Historique:
pmc-release:
24
01
2024
entrez:
23
2
2023
pubmed:
24
2
2023
medline:
24
2
2023
Statut:
ppublish
Résumé
Liver cultures may be used for disease modeling, testing therapies and predicting drug-induced injury. The complexity of the liver cultures has evolved from hepatocyte monocultures to co-cultures with non-parenchymal cells and finally to precision-cut liver slices. The latter culture format retains liver's native biomolecular and cellular complexity and therefore holds considerable promise for in vitro testing. However, liver slices remain functional for ~72 h in vitro and display limited utility for some disease modeling and therapy testing applications that require longer culture times. This paper describes a microfluidic device for longer-term maintenance of functional organotypic liver cultures. Our microfluidic culture system was designed to enable direct injection of liver tissue into a culture chamber through a valve-enabled side port. Liver tissue was embedded in collagen and remained functional for up to 31 days, highlighted by continued production of albumin and urea. These organotypic cultures also expressed several enzymes involved in xenobiotic metabolism. Conversely, matched liver tissue embedded in collagen in a 96-well plate lost its phenotype and function within 3-5 days. The microfluidic organotypic liver cultures described here represent a significant advance in liver cultivation and may be used for future modeling of liver diseases or for individualized liver-directed therapies.
Identifiants
pubmed: 36818276
doi: 10.1002/admt.202201121
pmc: PMC9937715
mid: NIHMS1840792
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK084567
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK107255
Pays : United States
Déclaration de conflit d'intérêts
Conflict of Interest The authors have no conflicts to disclose.
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