Long-read HiFi sequencing correctly assembles repetitive
Journal
GigaByte (Hong Kong, China)
ISSN: 2709-4715
Titre abrégé: GigaByte
Pays: China
ID NLM: 101775049
Informations de publication
Date de publication:
2022
2022
Historique:
received:
07
04
2022
accepted:
24
06
2022
entrez:
24
2
2023
pubmed:
25
2
2023
medline:
25
2
2023
Statut:
epublish
Résumé
Insect silk is a versatile biomaterial. Lepidoptera and Trichoptera display some of the most diverse uses of silk, with varying strength, adhesive qualities, and elastic properties. Silk fibroin genes are long (>20 Kbp), with many repetitive motifs that make them challenging to sequence. Most research thus far has focused on conserved N- and C-terminal regions of fibroin genes because a full comparison of repetitive regions across taxa has not been possible. Using the PacBio Sequel II system and SMRT sequencing, we generated high fidelity (HiFi) long-read genomic and transcriptomic sequences for the Indianmeal moth (
Identifiants
pubmed: 36824508
doi: 10.46471/gigabyte.64
pii: 64
pmc: PMC9693786
doi:
Types de publication
Journal Article
Langues
eng
Pagination
gigabyte64Informations de copyright
© The Author(s) 2022.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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