Detection, Visualization and Quantification of Protein Complexes in Human Alzheimer's Disease Brains using Proximity Ligation Assay.


Journal

Research square
Titre abrégé: Res Sq
Pays: United States
ID NLM: 101768035

Informations de publication

Date de publication:
16 Feb 2023
Historique:
entrez: 24 2 2023
pubmed: 25 2 2023
medline: 25 2 2023
Statut: epublish

Résumé

Examination of healthy and diseased human brain is essential to translational neuroscience. Protein-protein interactions play a pivotal role in physiological and pathological processes, but their detection is difficult, especially in aged and fixed human brain tissue. We used the proximity ligation assay (PLA) to broaden the range of molecular interactions assessable

Identifiants

pubmed: 36824944
doi: 10.21203/rs.3.rs-2570335/v1
pmc: PMC9949263
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIA NIH HHS
ID : K76 AG060001
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG078803
Pays : United States

Commentaires et corrections

Type : UpdateIn

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Auteurs

Wilber Romero-Fernandez (W)

Vanderbilt University Medical Center.

Cristian Carvajal-Tapia (C)

Vanderbilt University Medical Center.

Alex Prusky (A)

Vanderbilt University Medical Center.

Ketaki Katdare (K)

Vanderbilt University.

Emmeline Wang (E)

Vanderbilt University Medical Center.

Alena Shostak (A)

Vanderbilt University Medical Center.

Lissa Ventura-Antunes (L)

Vanderbilt University Medical Center.

Hannah Harmsen (H)

Vanderbilt University Medical Center.

Ethan Lippmann (E)

Vanderbilt University.

Dasiel Borroto-Escuela (D)

Karolinska Institute.

Jason MacGurn (J)

Vanderbilt University.

Kjell Fuxe (K)

Karolinska Institute.

Matthew Schrag (M)

Vanderbilt University Medical Center.

Classifications MeSH