Effect of the Enrichment in c-Kit Stem Cell Potential of Foetal Human Amniotic Fluid Cells: Characterization from Single Cell Analysis to the Secretome Content.

amniotic fluid stem cells sorting stemness transcriptome

Journal

Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304

Informations de publication

Date de publication:
02 Feb 2023
Historique:
received: 26 12 2022
revised: 30 01 2023
accepted: 31 01 2023
entrez: 25 2 2023
pubmed: 26 2 2023
medline: 26 2 2023
Statut: epublish

Résumé

Human amniotic fluid cells (hAFSCs) are a fascinating foetal cell-type that have important stem cell characteristics; however, they are a heterogeneous population that ranges from totally differentiated or progenitor cells to highly multipotent stem cells. There is no single approach to isolating the stem cell component, but the selection of a subpopulation of hAFSCs expressing c-Kit is widely employed, while a deep characterization of the two populations is still lacking. Here we performed single-cell and bulk RNAseq analysis to compare the gene expression profiles of adherent amniotic fluid cells and their subpopulation c-Kit

Identifiants

pubmed: 36830966
pii: biomedicines11020430
doi: 10.3390/biomedicines11020430
pmc: PMC9953071
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

Prenat Diagn. 2017 Nov;37(11):1093-1099
pubmed: 28842991
Mol Biosyst. 2015 Jun;11(6):1622-32
pubmed: 25811139
NPJ Regen Med. 2017 Oct 16;2:27
pubmed: 29302361
Aging (Albany NY). 2018 Oct 24;10(10):2911-2934
pubmed: 30362963
Cancer Lett. 2011 Apr 28;303(2):108-17
pubmed: 21320746
Cells. 2021 Jan 15;10(1):
pubmed: 33467440
Ital J Biochem. 2007 Dec;56(4):295-301
pubmed: 19192630
Placenta. 2013 Jul;34(7):526-35
pubmed: 23643069
Acta Histochem. 2017 Apr;119(3):315-326
pubmed: 28314612
Placenta. 2015 Jan;36(1):18-26
pubmed: 25468543
Biofactors. 2018 Mar;44(2):158-167
pubmed: 29341292
J Perinat Med. 2022 Aug 22;51(1):117-124
pubmed: 35985014
Oxid Med Cell Longev. 2018 Dec 17;2018:5263985
pubmed: 30647811
Signal Transduct Target Ther. 2022 Mar 21;7(1):92
pubmed: 35314676
Front Cell Dev Biol. 2020 Feb 28;8:87
pubmed: 32181250
J Biol Chem. 2016 Jul 29;291(31):15923-39
pubmed: 27252377
Cell Mol Life Sci. 2018 Sep;75(17):3159-3180
pubmed: 29947927
J Cell Sci. 2001 Mar;114(Pt 5):867-74
pubmed: 11181170
Cell. 2021 Jun 24;184(13):3573-3587.e29
pubmed: 34062119
Curr Pharm Des. 2012;18(13):1846-63
pubmed: 22352751
Arterioscler Thromb Vasc Biol. 2007 Apr;27(4):755-61
pubmed: 17272744
Mol Ther. 2012 Oct;20(10):1953-67
pubmed: 22760542
Front Cell Dev Biol. 2022 Jul 22;10:936990
pubmed: 35938174
Int J Biochem Cell Biol. 2022 Mar;144:106158
pubmed: 35063637
Int J Stem Cells. 2019 Mar 30;12(1):8-20
pubmed: 30836734
Nat Commun. 2018 Dec 3;9(1):5132
pubmed: 30510213
Neuron. 2017 Feb 8;93(3):560-573.e6
pubmed: 28111078
J Endod. 2020 Sep;46(9S):S56-S62
pubmed: 32950196
Stem Cells Int. 2019 Dec 27;2019:7869130
pubmed: 31949441
Reprod Biol. 2020 Sep;20(3):338-347
pubmed: 32518050
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Stem Cell Res Ther. 2015 Aug 22;6:150
pubmed: 26297012
Reprod Biomed Online. 2009;18 Suppl 1:17-27
pubmed: 19281660
Antioxidants (Basel). 2018 Jan 16;7(1):
pubmed: 29337889
Antioxid Redox Signal. 2014 Apr 20;20(12):1891-901
pubmed: 23621582
Nat Genet. 2008 May;40(5):499-507
pubmed: 18443585
Hematology. 2014 Mar;19(2):85-98
pubmed: 23710560
Cytotechnology. 2012 Oct;64(5):577-89
pubmed: 22410808
Organogenesis. 2012 Jul-Sep;8(3):77-88
pubmed: 23037870

Auteurs

Francesca Casciaro (F)

Department of Biomedical Sciences, University of Padua, 35131 Padova, Italy.

Francesca Beretti (F)

Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41124 Modena, Italy.

Martina Gatti (M)

Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41124 Modena, Italy.

Giuseppe Persico (G)

Department of Experimental Oncology, IRCCS-European Institute of Oncology, 20139 Milano, Italy.

Emma Bertucci (E)

Department of Medical and Surgical Sciences for Mothers, Children and Adults, University of Modena and Reggio Emilia, Azienda Ospedaliero Universitaria Policlinico, 41124 Modena, Italy.

Marco Giorgio (M)

Department of Biomedical Sciences, University of Padua, 35131 Padova, Italy.
Department of Experimental Oncology, IRCCS-European Institute of Oncology, 20139 Milano, Italy.

Tullia Maraldi (T)

Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, 41124 Modena, Italy.

Classifications MeSH