RNAseq-Based Working Model for Transcriptional Regulation of Crosstalk between Simultaneous Abiotic UV-B and Biotic Stresses in Plants.


Journal

Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097

Informations de publication

Date de publication:
17 01 2023
Historique:
received: 19 12 2022
revised: 10 01 2023
accepted: 14 01 2023
entrez: 25 2 2023
pubmed: 26 2 2023
medline: 3 3 2023
Statut: epublish

Résumé

Plants adjust their secondary metabolism by altering the expression of corresponding genes to cope with both abiotic and biotic stresses. In the case of UV-B radiation, plants produce protective flavonoids; however, this reaction is impeded during pattern-triggered immunity (PTI) induced by pathogens. Pathogen attack can be mimicked by the application of microbial associated molecular patterns (e.g., flg22) to study crosstalk between PTI and UV-B-induced signaling pathways. Switching from Arabidopsis cell cultures to in planta studies, we analyzed whole transcriptome changes to gain a deeper insight into crosstalk regulation. We performed a comparative transcriptomic analysis by RNAseq with four distinct mRNA libraries and identified 10778, 13620, and 11294 genes, which were differentially expressed after flg22, UV-B, and stress co-treatment, respectively. Focusing on genes being either co-regulated with the UV-B inducible marker gene chalcone synthase

Identifiants

pubmed: 36833168
pii: genes14020240
doi: 10.3390/genes14020240
pmc: PMC9957429
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Zheng Zhou (Z)

Department of Molecular Phytopathology and Biotechnology, Institute of Phytopathology, Christian-Albrechts-Universität zu Kiel, 24118 Kiel, Germany.

Alex Windhorst (A)

Plant Breeding Methodology, Georg-August-University Göttingen, 37073 Göttingen, Germany.

Dirk Schenke (D)

Department of Molecular Phytopathology and Biotechnology, Institute of Phytopathology, Christian-Albrechts-Universität zu Kiel, 24118 Kiel, Germany.

Daguang Cai (D)

Department of Molecular Phytopathology and Biotechnology, Institute of Phytopathology, Christian-Albrechts-Universität zu Kiel, 24118 Kiel, Germany.

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Classifications MeSH