The Distinction between Dematiaceous Molds and Non-Dematiaceous Fungi in Clinical and Spiked Samples Treated with Hydrogen Peroxide Using Direct Fluorescence Microscopy.
black fungi
dematiaceous molds
fluorescence microscopy
hydrogen peroxide
melanin
Journal
Journal of fungi (Basel, Switzerland)
ISSN: 2309-608X
Titre abrégé: J Fungi (Basel)
Pays: Switzerland
ID NLM: 101671827
Informations de publication
Date de publication:
09 Feb 2023
09 Feb 2023
Historique:
received:
12
01
2023
revised:
01
02
2023
accepted:
07
02
2023
entrez:
25
2
2023
pubmed:
26
2
2023
medline:
26
2
2023
Statut:
epublish
Résumé
Dematiaceous fungi are pigmented molds with a high content of melanin in their cell walls that can cause fatal infections in immunocompromised hosts. Direct microscopy is the main method for the rapid diagnosis of dematiaceous fungi in clinical specimens. However, it is often difficult to distinguish their hyphae from non-dematiaceous hyphae and yeast pseudohyphae. Our aim was to develop a fluorescence staining method that targets melanin for the detection of dematiaceous molds in clinical specimens. Glass slide smears of clinical samples and sterile bronchoalveolar lavage spiked with dematiaceous and non-dematiaceous fungi were treated with hydrogen peroxide, and digital images were recorded using direct microscopy with different fluorescent filters. The images of fungi were compared for their fluorescence intensity using the NIS-Elements software. The fluorescent signal between dematiaceous and non-dematiaceous fungi demonstrated a markedly increased mean intensity for dematiaceous molds following hydrogen peroxide treatment (7510.3 ± 10,427.6 vs. 0.3 ± 3.1, respectively,
Identifiants
pubmed: 36836341
pii: jof9020227
doi: 10.3390/jof9020227
pmc: PMC9967646
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Biomed Opt Express. 2019 Jan 09;10(2):500-513
pubmed: 30800495
J Gen Microbiol. 1988 Apr;134(4):1037-41
pubmed: 3141580
Clin Infect Dis. 2002 Feb 15;34(4):467-76
pubmed: 11797173
Histochem J. 2001 May;33(5):273-81
pubmed: 11563540
J Fungi (Basel). 2015 Dec 22;2(1):
pubmed: 29376919
Free Radic Biol Med. 2017 Jul;108:110-117
pubmed: 28323131
Invest Ophthalmol Vis Sci. 2001 Jan;42(1):241-6
pubmed: 11133875
Bone Marrow Transplant. 2012 Aug;47(8):1030-45
pubmed: 22231461
J Antimicrob Chemother. 2002 Nov;50(5):719-22
pubmed: 12407129
Clin Microbiol Rev. 2010 Oct;23(4):884-928
pubmed: 20930077
J Biol Chem. 2022 Jan;298(1):101519
pubmed: 34942148
Semin Respir Crit Care Med. 2020 Feb;41(1):131-140
pubmed: 32000289
J Fungi (Basel). 2019 Jul 10;5(3):
pubmed: 31295828
Antimicrob Agents Chemother. 2017 Feb 23;61(3):
pubmed: 28031206