A Multimodal Desorption Electrospray Ionisation Workflow Enabling Visualisation of Lipids and Biologically Relevant Elements in a Single Tissue Section.
biological tissue analysis
correlative imaging
desorption electrospray ionisation mass spectrometry
ion beam analysis
multimodal imaging
Journal
Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790
Informations de publication
Date de publication:
11 Feb 2023
11 Feb 2023
Historique:
received:
25
01
2023
revised:
07
02
2023
accepted:
09
02
2023
entrez:
25
2
2023
pubmed:
26
2
2023
medline:
26
2
2023
Statut:
epublish
Résumé
The colocation of elemental species with host biomolecules such as lipids and metabolites may shed new light on the dysregulation of metabolic pathways and how these affect disease pathogeneses. Alkali metals have been the subject of extensive research, are implicated in various neurodegenerative and infectious diseases and are known to disrupt lipid metabolism. Desorption electrospray ionisation (DESI) is a widely used approach for molecular imaging, but previous work has shown that DESI delocalises ions such as potassium (K) and chlorine (Cl), precluding the subsequent elemental analysis of the same section of tissue. The solvent typically used for the DESI electrospray is a combination of methanol and water. Here we show that a novel solvent system, (50:50 (%
Identifiants
pubmed: 36837881
pii: metabo13020262
doi: 10.3390/metabo13020262
pmc: PMC9964958
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Engineering and Physical Sciences Research Council
ID : EP/R031118/1
Organisme : Analytical Chemistry Trust Fund
ID : 600310/10
Organisme : Natural Environment Research Council
ID : NE/V017616/1
Organisme : Engineering and Physical Sciences Research Council
ID : NS/A000059/1
Organisme : Horizon 2020
ID : 824096
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