rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis.
dogs
humans
leishmaniasis
recombinant chimeric protein
serodiagnosis
visceral leishmaniasis
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
11 Feb 2023
11 Feb 2023
Historique:
received:
07
12
2022
revised:
08
02
2023
accepted:
09
02
2023
entrez:
25
2
2023
pubmed:
26
2
2023
medline:
26
2
2023
Statut:
epublish
Résumé
visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.
Sections du résumé
BACKGROUND
BACKGROUND
visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem.
METHODS
METHODS
a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of
RESULTS
RESULTS
in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA).
CONCLUSIONS
CONCLUSIONS
the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.
Identifiants
pubmed: 36839574
pii: pathogens12020302
doi: 10.3390/pathogens12020302
pmc: PMC9958779
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Vet Parasitol. 2013 Sep 23;196(3-4):296-300
pubmed: 23562649
J Clin Lab Anal. 2018 Jul;32(6):e22410
pubmed: 29453831
Cell Stress Chaperones. 2002 Oct;7(4):339-46
pubmed: 12653478
Trans R Soc Trop Med Hyg. 2012 Jan;106(1):54-9
pubmed: 22137538
Clin Exp Immunol. 1987 Apr;68(1):78-85
pubmed: 3115642
Immunol Lett. 1998 Oct;63(3):169-74
pubmed: 9840686
J Infect Dis. 1996 Mar;173(3):758-61
pubmed: 8627048
NPJ Vaccines. 2020 Aug 13;5:75
pubmed: 32821440
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):775-9
pubmed: 8421715
Vet Parasitol. 2021 Aug;296:109513
pubmed: 34225189
Infect Immun. 2003 Jul;71(7):3988-94
pubmed: 12819086
Int Immunopharmacol. 2019 Nov;76:105872
pubmed: 31499268
Parasit Vectors. 2014 Mar 24;7:111
pubmed: 24655335
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
PLoS Negl Trop Dis. 2020 Oct 8;14(10):e0008741
pubmed: 33031382
Ann Trop Med Parasitol. 1995 Dec;89 Suppl 1:89-93
pubmed: 8745931
Biochem Biophys Res Commun. 2021 Feb 12;540:37-41
pubmed: 33429198
Clin Diagn Lab Immunol. 2002 Sep;9(5):951-8
pubmed: 12204943
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Parasit Vectors. 2018 Jun 28;11(1):372
pubmed: 29954428
Clin Diagn Lab Immunol. 1998 Sep;5(5):717-20
pubmed: 9729541
Vet J. 2019 Nov;253:105387
pubmed: 31685139
PLoS One. 2013 Jul 26;8(7):e69988
pubmed: 23922884
Recent Pat Biotechnol. 2019;13(4):316-328
pubmed: 31333134
Am J Trop Med Hyg. 1996 Aug;55(2):125-30
pubmed: 8780448
Pathog Glob Health. 2015 Sep;109(6):283-9
pubmed: 26257311
Vet Med Int. 2011;2011:215964
pubmed: 21772963
Epidemiol Infect. 2017 Sep;145(12):2436-2444
pubmed: 28726597
Trans R Soc Trop Med Hyg. 1994 Sep-Oct;88(5):543-5
pubmed: 7992333
PLoS Negl Trop Dis. 2019 Jul 18;13(7):e0007484
pubmed: 31318856
Arch Razi Inst. 2020 Oct;75(3):327-338
pubmed: 33025773
PLoS Negl Trop Dis. 2011 Sep;5(9):e1310
pubmed: 21931874
J Parasitol. 1995 Dec;81(6):1000-3
pubmed: 8544037
PLoS Negl Trop Dis. 2015 Jan 08;9(1):e3429
pubmed: 25569685
Biomed Res Int. 2013;2013:148317
pubmed: 24294596
Iran J Parasitol. 2021 Jul-Sep;16(3):377-385
pubmed: 34630582
J Vector Borne Dis. 2021 Oct-Dec;58(4):289-296
pubmed: 35381816
Protein Expr Purif. 2006 May;47(1):319-28
pubmed: 16504539
Mol Cell Proteomics. 2007 Jan;6(1):88-101
pubmed: 17050524
PLoS One. 2012;7(5):e35671
pubmed: 22693548
Immunol Lett. 2001 Mar 1;76(2):107-10
pubmed: 11274727
Cad Saude Publica. 2020 Jun 08;36(6):e00060219
pubmed: 32520123
Trans R Soc Trop Med Hyg. 2000 Sep-Oct;94(5):484-7
pubmed: 11132371
Iran J Parasitol. 2021 Jan-Mar;16(1):1-10
pubmed: 33786042
Am J Trop Med Hyg. 1995 Mar;52(3):287-92
pubmed: 7694973
Hepat Res Treat. 2016;2016:6592143
pubmed: 26942007
Immunol Lett. 1996 Sep;52(2-3):73-9
pubmed: 8905399
Exp Parasitol. 2019 Apr;199:9-16
pubmed: 30796913
Vet Parasitol. 2002 Apr 2;104(4):275-85
pubmed: 11836028
Infect Immun. 1995 Oct;63(10):4105-14
pubmed: 7558326
Iran Biomed J. 2016 Jul;20(3):128-34
pubmed: 26883952
Methods Enzymol. 1973;27:675-735
pubmed: 4797940
Iran J Immunol. 2009 Jun;6(2):75-86
pubmed: 19561376
Diagnostics (Basel). 2022 Jun 11;12(6):
pubmed: 35741250
Iran J Immunol. 2011 Dec;8(4):244-50
pubmed: 22201622