High throughput assay for compounds that boost BDNF expression in neurons.

Mice Animals Brain-Derived Neurotrophic Factor / genetics High-Throughput Screening Assays Neurons / metabolism Calcium Channels, L-Type / metabolism Brain / metabolism
Brain-derived neurotrophic factor Gene expression HTS assay Knockin LOPAC library Nano luciferase

Journal

SLAS discovery : advancing life sciences R & D
ISSN: 2472-5560
Titre abrégé: SLAS Discov
Pays: United States
ID NLM: 101697563

Informations de publication

Date de publication:
04 2023
Historique:
received: 02 12 2022
revised: 30 01 2023
accepted: 21 02 2023
medline: 25 4 2023
pubmed: 27 2 2023
entrez: 26 2 2023
Statut: ppublish

Résumé

Deficiencies in brain-derived neurotrophic factor (BDNF) have been linked to several brain disorders, making compounds that can boost neuronal BDNF synthesis attractive as potential therapeutics. However, a sensitive and quantitative BDNF assay for high-throughput screening (HTS) is still missing. Here we report the generation of a new mouse Bdnf allele, Bdnf

Identifiants

DOI: 10.1016/j.slasd.2023.02.005 PMID: 36842668
pubmed: 36842668
pii: S2472-5552(23)00018-7
doi: 10.1016/j.slasd.2023.02.005
pii:
doi:

Substances chimiques

Brain-Derived Neurotrophic Factor 0
Calcium Channels, L-Type 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

88-94

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK103335
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK105954
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH125187
Pays : United States

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare no competing interests.

Auteurs

Guey-Ying Liao (GY)

Department of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA.

Haifei Xu (H)

Department of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA.

Justin Shumate (J)

Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA.

Louis Scampavia (L)

Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA.

Timothy Spicer (T)

Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA.

Baoji Xu (B)

Department of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, University of Florida, 130 Scripps Way, Jupiter, FL 33458, USA. Electronic address: baoji.xu@ufl.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › High throughput assay for compounds that boost...
questionsmedicales.fr › Eucaryotes › Animaux › High throughput assay for compounds that boost...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Facteurs de croissance nerveuse › Facteur neurotrophique dérivé du cerveau › High throughput assay for compounds that boost...
questionsmedicales.fr › Techniques d'investigation › Tests de criblage à haut débit › High throughput assay for compounds that boost...
questionsmedicales.fr › Cellules › Neurones › High throughput assay for compounds that boost...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux calciques › Canaux calciques de type L › High throughput assay for compounds that boost...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › High throughput assay for compounds that boost...