Detection of Zebrafish Retinal Proteins by Infrared Western Blotting.
Circadian rhythms
Infrared fluorescence western blot
Retina
Visual transduction
Zebrafish
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Feb 2023
20 Feb 2023
Historique:
received:
21
07
2022
revised:
29
09
2022
accepted:
23
01
2023
entrez:
27
2
2023
pubmed:
28
2
2023
medline:
28
2
2023
Statut:
epublish
Résumé
The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research. This protocol describes a method to quantitatively evaluate the expression of Arrestin3a (Arr3a) and G-protein receptor kinase7a (Grk7a) in the adult zebrafish retina at protein levels by infrared fluorescence western blot. Our protocol can be easily adapted to measure protein levels in additional zebrafish tissues.
Identifiants
pubmed: 36845534
doi: 10.21769/BioProtoc.4618
pii: e4618
pmc: PMC9947546
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4618Informations de copyright
Copyright © 2023 The Authors; This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
Déclaration de conflit d'intérêts
Competing interestsThe authors have no conflict of interest.
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