Fecal identification markers impact the feline fecal microbiota.
16S rRNA
amplicon sequencing
fecal marker
fecal microbiota
fecal sample
feline microbiota
veterinary medicine
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2023
2023
Historique:
received:
08
09
2022
accepted:
02
01
2023
entrez:
27
2
2023
pubmed:
28
2
2023
medline:
28
2
2023
Statut:
epublish
Résumé
Fecal diagnostics are a mainstay of feline medicine, and fecal identification markers help to distinguish individuals in a multi-cat environment. However, the impact of identification markers on the fecal microbiota are unknown. Given the increased interest in using microbiota endpoints to inform diagnosis and treatment, the objective of this study was to examine the effects of orally supplemented glitter and crayon shavings on the feline fecal microbiota (amplicon sequencing of 16S rRNA gene V4 region). Fecal samples were collected daily from six adult cats that were randomized to receive oral supplementation with either glitter or crayon for two weeks, with a two-week washout before receiving the second marker. No adverse effects in response to marker supplementation were seen for any cat, and both markers were readily identifiable in the feces. Microbiota analysis revealed idiosyncratic responses to fecal markers, where changes in community structure in response to glitter or crayon could not be readily discerned. Given these findings, it is not recommended to administered glitter or crayon shavings as a fecal marker when microbiome endpoints are used, however their clinical use with other diagnostics should still be considered.
Identifiants
pubmed: 36846255
doi: 10.3389/fvets.2023.1039931
pmc: PMC9946173
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1039931Informations de copyright
Copyright © 2023 Nealon, Wood, Rudinsky, Klein, Salerno, Parker, Quimby, Howard and Winston.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
J Vet Intern Med. 2019 Mar;33(2):662-669
pubmed: 30561098
J Am Vet Med Assoc. 1999 May 1;214(9):1336-41
pubmed: 10319174
Nutr Res Rev. 2014 Dec;27(2):295-307
pubmed: 25623083
Behav Brain Res. 2021 Sep 24;414:113474
pubmed: 34280457
Brain Behav Immun. 2011 Mar;25(3):397-407
pubmed: 21040780
Appl Environ Microbiol. 2020 Mar 18;86(7):
pubmed: 32005736
J Feline Med Surg. 2022 Feb;24(2):131-141
pubmed: 34013812
PLoS One. 2014 Oct 03;9(10):e108729
pubmed: 25279695
PLoS One. 2020 Mar 18;15(3):e0230239
pubmed: 32187226
Appl Environ Microbiol. 2013 Sep;79(17):5112-20
pubmed: 23793624
Microb Biotechnol. 2020 Nov;13(6):1708-1732
pubmed: 32864871
PLoS One. 2015 May 19;10(5):e0127378
pubmed: 25992741
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Vet Clin North Am Small Anim Pract. 2011 Mar;41(2):261-72
pubmed: 21486635
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
Nat Commun. 2021 Nov 18;12(1):6740
pubmed: 34795283
J Proteome Res. 2012 Dec 7;11(12):5924-33
pubmed: 23075436
Bioinformatics. 2015 Aug 1;31(15):2461-8
pubmed: 25819674
Genome Biol. 2014;15(12):550
pubmed: 25516281
Gut Microbes. 2014 May-Jun;5(3):390-6
pubmed: 24690880
Vet Microbiol. 2019 Apr;231:197-206
pubmed: 30955810
J Feline Med Surg. 2022 Jun;24(6):e1-e12
pubmed: 35266809
J Anim Sci. 2010 Sep;88(9):2978-87
pubmed: 20495116
Clin Tech Small Anim Pract. 2004 Nov;19(4):216-24
pubmed: 18371318
Sci Rep. 2019 Dec 16;9(1):19208
pubmed: 31844119
Front Microbiol. 2017 Nov 15;8:2224
pubmed: 29187837
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
pubmed: 20534432
PLoS One. 2012;7(12):e52078
pubmed: 23284876
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
FEMS Microbiol Ecol. 2008 Dec;66(3):590-8
pubmed: 19049654
Front Microbiol. 2020 Jun 25;11:1266
pubmed: 32670224
J Nutr. 1994 Dec;124(12 Suppl):2663S-2669S
pubmed: 7996263
Front Microbiol. 2020 Jul 24;11:1661
pubmed: 32793152
Br J Nutr. 2017 Oct;118(7):513-524
pubmed: 28958218
Vet Clin Pathol. 2022 Feb;50 Suppl 1:6-17
pubmed: 34514619
Contemp Top Lab Anim Sci. 2002 Mar;41(2):51-6
pubmed: 11958604
PLoS One. 2021 Dec 15;16(12):e0253031
pubmed: 34910719
J Anim Physiol Anim Nutr (Berl). 2001 Jun;85(5-6):135-41
pubmed: 11686781
J Feline Med Surg. 2012 Oct;14(10):669-77
pubmed: 22577048
BMC Vet Res. 2017 Aug 18;13(1):256
pubmed: 28821259