The effect of miR-372-5p regulation on CDX1 and CDX2 in the gastric cancer cell line.
AGS cell line
CDX1
CDX2
gastric cancer
miR-372-5p
Journal
Hormone molecular biology and clinical investigation
ISSN: 1868-1891
Titre abrégé: Horm Mol Biol Clin Investig
Pays: Germany
ID NLM: 101538885
Informations de publication
Date de publication:
01 Sep 2023
01 Sep 2023
Historique:
received:
08
05
2022
accepted:
08
02
2023
medline:
2
10
2023
pubmed:
28
2
2023
entrez:
27
2
2023
Statut:
epublish
Résumé
MicroRNA expression disruptions play an important function in the expansion of gastric cancer. Previous investigation has indicated that miR-372-5p doing as an oncogene in several malignancies. CDX1 and CDX2, as target genes of miR-372-5p, play the role of tumor suppressors and oncogenes in gastric cancer cells, respectively. The current investigation explored the effects of miR-372-5p regulation on CDX2 and CDX1 in AGS cell lines and studied their molecular mechanism. hsa-miR-372-5p miRCURY LNA miRNA Inhibitors and Mimic were transfected into AGS cell line. The cell viability and cell cycle calculation were defined by MTT assay and flow cytometry, respectively. The Expression levels of miR-372-5p, CDX1, CDX2 and transfection efficiency were measured using Real-time PCR. Statistical investigation p values <0.05 were considered to be meaningful. miR-372-5p particularly was upregulated in control cells and also after transfection by mimic. While its expression was reduced by the inhibitor. Upregulation of miR-372-5p remarkably increased cell growth and led to accumulation in the G2/M phase, although the inhibitor decreased cell growth and accumulation in the S phase. Accordingly, upregulation of miR-372-5p increased CDX2 and decreased CDX1 expression. By inhibition of miR-372-5p, expression of CDX2 was decreased and expression of CDX1 was increased. Up and down-regulation of miR-372-5P has a potential effect on the expression levels of its target genes, CDX1 and CDX22. Accordingly, the downregulation of miR-372-5p may be assumed as a possible therapeutic target in treating gastric cancer.
Identifiants
pubmed: 36848481
pii: hmbci-2022-0045
doi: 10.1515/hmbci-2022-0045
doi:
Substances chimiques
CDX1 protein, human
0
CDX2 protein, human
0
CDX2 Transcription Factor
0
Homeodomain Proteins
0
MicroRNAs
0
MIRN372 microRNA, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
271-276Informations de copyright
© 2023 Walter de Gruyter GmbH, Berlin/Boston.
Références
Zhang, X, Xie, K, Zhou, H, Wu, Y, Li, C, Liu, Y, et al.. Role of non-coding RNAs and RNA modifiers in cancer therapy resistance. Mol Cancer 2020;19:1–26. https://doi.org/10.1186/s12943-020-01171-z .
doi: 10.1186/s12943-020-01171-z
Abbaszadegan, MR, Mojarrad, M, Rahimi, HR, Moghbeli, M. Genetic and molecular biology of gastric cancer among Iranian patients: an update. Egypt J Med Hum Genet 2022;23:1–13. https://doi.org/10.1186/s43042-022-00232-w .
doi: 10.1186/s43042-022-00232-w
Shafaghi, A, Gharibpoor, F, Mahdipour, Z, Samadani, AA. Comparison of three risk scores to predict outcomes in upper gastrointestinal bleeding; modifying Glasgow–Blatchford with albumin. Rom J Intern Med 2019;57:322–33. https://doi.org/10.2478/rjim-2019-0016 .
doi: 10.2478/rjim-2019-0016
Ala, U. Competing endogenous RNAs, non-coding RNAs and diseases: an intertwined story. Cells 2020;9:1574. https://doi.org/10.3390/cells9071574 .
doi: 10.3390/cells9071574
O’Brien, J, Hayder, H, Zayed, Y, Peng, C. Overview of microRNA biogenesis, mechanisms of actions, and circulation. Front Endocrinol 2018;9:402. https://doi.org/10.3389/fendo.2018.00402 .
doi: 10.3389/fendo.2018.00402
Mirzaei, S, Gholami, MH, Hushmandi, K, Hashemi, F, Zabolian, A, Canadas, I, et al.. The long and short non-coding RNAs modulating EZH2 signaling in cancer. J Hematol Oncol 2022;15:18. https://doi.org/10.1186/s13045-022-01235-1 .
doi: 10.1186/s13045-022-01235-1
Cao, H, Feng, Y, Chen, L. Repression of micro RNA‐372 by arsenic sulphide inhibits prostate cancer cell proliferation and migration through regulation of large tumour suppressor kinase 2. Basic Clin Pharmacol Toxicol 2017;120:256-63, https://doi.org/10.1111/bcpt.12687 .
doi: 10.1111/bcpt.12687
Cho, WJ, Shin, JM, Kim, JS, Lee, MR, Hong, KS, Lee, JH, et al.. miR-372 regulates cell cycle and apoptosis of ags human gastric cancer cell line through direct regulation of LATS2. Mol Cell 2009;28:521–7. https://doi.org/10.1007/s10059-009-0158-0 .
doi: 10.1007/s10059-009-0158-0
Wu, CC, Hsu, TW, Yeh, CC, Huang, HB. The role of transcription factor caudal-related homeobox transcription factor 2 in colorectal cancer. Tzu Chi Med J 2020;32:305. https://doi.org/10.4103/tcmj.tcmj_49_20 .
doi: 10.4103/tcmj.tcmj_49_20
Tsukamoto, T, Mizoshita, T, Mihara, M, Tanaka, H, Takenaka, Y, Yamamura, Y, et al.. Sox2 expression in human stomach adenocarcinomas with gastric and gastric‐and‐intestinal‐mixed phenotypes. Histopathology 2005;46:649–58. https://doi.org/10.1111/j.1365-2559.2005.02170.x .
doi: 10.1111/j.1365-2559.2005.02170.x
Wang, Y, Li, Z, Li, W, Liu, S, Han, B. Methylation of promoter region of CDX2 gene in colorectal cancer. Oncol Lett 2016;12:3229–33. https://doi.org/10.3892/ol.2016.5109 .
doi: 10.3892/ol.2016.5109
Peng, Y, Guo, JJ, Liu, YM, Wu, XL. MicroRNA-34A inhibits the growth, invasion and metastasis of gastric cancer by targeting PDGFR and MET expression. Biosci Rep 2014;34:e00112. https://doi.org/10.1042/bsr20140020 .
doi: 10.1042/bsr20140020
Nakayama, C, Yamamichi, N, Tomida, S, Takahashi, Y, Kageyama‐Yahara, N, Sakurai, K, et al.. Transduced caudal‐type homeobox (CDX) 2/CDX 1 can induce growth inhibition on CDX‐deficient gastric cancer by rapid intestinal differentiation. Cancer Sci 2018;109:3853–64. https://doi.org/10.1111/cas.13821 .
doi: 10.1111/cas.13821
Cheng, X, Chen, J, Huang, Z. miR-372 promotes breast cancer cell proliferation by directly targeting LATS2. Exp Ther Med . 2018;15:2812-7, https://doi.org/10.3892/etm.2018.5761 .
doi: 10.3892/etm.2018.5761
Wu, G, Wang, Y, Lu, X, He, H, Liu, H, Meng, X, et al.. Low mir-372 expression correlates with poor prognosis and tumor metastasis in hepatocellular carcinoma. BMC Cancer 2015;15:1–12. https://doi.org/10.1186/s12885-015-1214-0 .
doi: 10.1186/s12885-015-1214-0
Zhou, C, Li, X, Zhang, X, Liu, X, Tan, Z, Yang, C, et al.. microRNA-372 maintains oncogene characteristics by targeting TNFAIP1 and affects NFκB signaling in human gastric carcinoma cells. Int J Oncol 2013;42:635–42. https://doi.org/10.3892/ijo.2012.1737 .
doi: 10.3892/ijo.2012.1737
Wang, Q, Liu, S, Zhao, X, Wang, Y, Tian, D, Jiang, W. MiR‐372‐3p promotes cell growth and metastasis by targeting FGF 9 in lung squamous cell carcinoma. Cancer Med 2017;6:1323–30. https://doi.org/10.1002/cam4.1026 .
doi: 10.1002/cam4.1026
Jafari, N, Abediankenari, S, Hosseini-Khah, Z, Valizadeh, SM, Torabizadeh, Z, Zaboli, E, et al.. Expression patterns of seven key genes, including β-catenin, Notch1, GATA6, CDX2, miR-34a, miR-181a and miR-93 in gastric cancer. Sci Rep 2020;10:1–16. https://doi.org/10.1038/s41598-020-69308-0 .
doi: 10.1038/s41598-020-69308-0
Zhang, JF, Qu, LS, Qian, XF, Xia, BL, Mao, ZB, Chen, WC. Nuclear transcription factor CDX2 inhibits gastric cancer-cell growth and reverses epithelial-to-mesenchymal transition in vitro and in vivo. Mol Med Rep 2015;12:5231–8. https://doi.org/10.3892/mmr.2015.4114 .
doi: 10.3892/mmr.2015.4114
Kiani, S, Akhavan-Niaki, H, Fattahi, S, Kavoosian, S, Jelodar, NB, Bagheri, N, et al.. Purified sulforaphane from broccoli (Brassica oleracea var. italica) leads to alterations of CDX1 and CDX2 expression and changes in miR-9 and miR-326 levels in human gastric cancer cells. Gene 2018;678:115–23. https://doi.org/10.1016/j.gene.2018.08.026 .
doi: 10.1016/j.gene.2018.08.026
Li, T, Lu, Y, Zhao, X, Guo, H, Liu, C, Li, H, et al.. MicroRNA-296-5p increases proliferation in gastric cancer through repression of Caudal-related homeobox 1. Oncogene 2014;33:783–93. https://doi.org/10.1038/onc.2012.637 .
doi: 10.1038/onc.2012.637
Samadani, AA, Keymoradzdeh, A, Shams, S, Soleymanpour, A, Norollahi, SE, Vahidi, S, et al.. Mechanisms of cancer stem cell therapy. Clin Chim Acta 2020;510:581–92. https://doi.org/10.1016/j.cca.2020.08.016 .
doi: 10.1016/j.cca.2020.08.016
Norollahi, SE, Mansour-Ghanaei, F, Joukar, F, Ghadarjani, S, Mojtahedi, K, Nejad, KG, et al.. Therapeutic approach of Cancer stem cells (CSCs) in gastric adenocarcinoma; DNA methyltransferases enzymes in cancer targeted therapy. Biomed Pharmacother 2019;115:108958. https://doi.org/10.1016/j.biopha.2019.108958 .
doi: 10.1016/j.biopha.2019.108958