Designing a turn-on ultrasensitive fluorescent probe based on ICT-FRET for detection and bioimaging of Hypochlorous acid.

Animals Mice Fluorescence Resonance Energy Transfer Hypochlorous Acid Fluorescent Dyes Optical Imaging / methods RAW 264.7 Cells
Bioimaging Fluorescent probe Hypochlorous acid ICT-FRET dual-mechanism TD-DFT calculation

Journal

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
ISSN: 1873-3557
Titre abrégé: Spectrochim Acta A Mol Biomol Spectrosc
Pays: England
ID NLM: 9602533

Informations de publication

Date de publication:
05 Jun 2023
Historique:
received: 23 10 2022
revised: 12 02 2023
accepted: 20 02 2023
medline: 4 4 2023
pubmed: 28 2 2023
entrez: 27 2 2023
Statut: ppublish

Résumé

Hypochlorous acid (HClO) plays an essential role in biological systems. The characteristics of potent oxidization and short lifetime make it challenging to detect specifically from other reactive oxygen species (ROS) at cellular levels. Therefore, its detection and imaging with high selectivity and sensitivity are of great significance. Herein a turn-on HClO fluorescent probe (named RNB-OCl) with boronate ester as the recognition site was designed and synthesized. The RNB-OCl displayed good selective and ultrasensitive to HClO with a low detection limit of 1.36 nM by the intramolecular charge transfer (ICT)-fluorescence resonance energy transfer (FRET) dual mechanism in reducing the fluorescence background and improving the sensitivity. In addition, the role of the ICT-FRET was further demonstrated by time-dependent density functional theory (TD-DFT) calculations. Furthermore, the probe RNB-OCl was successfully employed for imaging HClO in living cells.

Identifiants

DOI: 10.1016/j.saa.2023.122546 PMID: 36848857
pubmed: 36848857
pii: S1386-1425(23)00231-7
doi: 10.1016/j.saa.2023.122546
pii:
doi:

Substances chimiques

Hypochlorous Acid 712K4CDC10
Fluorescent Dyes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122546

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Jihua Zhu (J)

College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining, Qinghai 810008, PR China; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China; Academy of Plateau Science and Sustainability, People's Government of Qinghai Province & Beijing Normal University, Xining, Qinghai 810008, P. R. China.

Congcong Miao (C)

College of Chemistry and Chemical Engineering, Qinghai Normal University, Xining, Qinghai 810008, PR China; College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China.

Xicun Wang (X)

College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, P. R. China. Electronic address: wangxicun@nwnu.edu.cn.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Acide hypochloreux › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Techniques d'investigation › Imagerie optique › Designing a turn-on ultrasensitive fluorescent...
questionsmedicales.fr › Cellules › Cellules RAW 264.7 › Designing a turn-on ultrasensitive fluorescent...