Protective effect of bone morphogenetic protein-7 induced differentiation of bone marrow mesenchymal stem cells in rat with acute spinal cord injury.
Acute spinal cord injury
BMP-7
BMSCs
Neuroregeneration
Journal
Functional & integrative genomics
ISSN: 1438-7948
Titre abrégé: Funct Integr Genomics
Pays: Germany
ID NLM: 100939343
Informations de publication
Date de publication:
27 Feb 2023
27 Feb 2023
Historique:
received:
24
12
2022
accepted:
17
02
2023
revised:
08
02
2023
entrez:
27
2
2023
pubmed:
28
2
2023
medline:
3
3
2023
Statut:
epublish
Résumé
The principal aim of present study was to assess the therapeutic efficacy of bone morphogenetic protein-7 (BMP-7) induced differentiation of bone marrow mesenchymal stem cells (BMSCs) in a rat acute spinal cord injury (SCI) model. BMSCs were isolated from rats, and then divided into a control and a BMP-7 induction groups. The proliferation ability of BMSCs and glial cell markers were determined. Forty Sprague-Dawley (SD) rats were randomly divided into sham, SCI, BMSC, and BMP7 + BMSC groups (n = 10). Among these rats, the recovery of hind limb motor function, the pathological related markers, and motor evoked potentials (MEP) were identified. BMSCs differentiated into neuron-like cells after the introduction of exogenous BMP-7. Interestingly, the expression levels of MAP-2 and Nestin increased, whereas the expression level of GFAP decreased after the treatment with exogenous BMP-7. Furthermore, the Basso, Beattie, and Bresnahan (BBB) score reached 19.33 ± 0.58 in the BMP-7 + BMSC group at day 42. Nissl bodies in the model group were reduced compared to the sham group. After 42 days, in both the BMSC and BMP-7 + BMSC groups, the number of Nissl bodies increased. This is especially so for the number of Nissl bodies in the BMP-7 + BMSC group, which was more than that in the BMSC group. The expression of Tuj-1 and MBP in BMP-7 + BMSC group increased, whereas the expression of GFAP decreased. Moreover, the MEP waveform decreased significantly after surgery. Furthermore, the waveform was wider and the amplitude was higher in BMP-7 + BMSC group than that in BMSC group. BMP-7 promotes BMSC proliferation, induces the differentiation of BMSCsinto neuron-like cells, and inhibits the formation of glial scar. BMP-7 plays a confident role in the recovery of SCI rats.
Identifiants
pubmed: 36849554
doi: 10.1007/s10142-023-00994-5
pii: 10.1007/s10142-023-00994-5
doi:
Substances chimiques
Bone Morphogenetic Protein 7
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
68Subventions
Organisme : China Natural Science Foundation
ID : 81560216
Organisme : China Natural Science Foundation
ID : 81560216
Organisme : China Natural Science Foundation
ID : 81560216
Organisme : China Natural Science Foundation
ID : 81560216
Organisme : China Natural Science Foundation
ID : 81560216
Organisme : Open Topics of the Department level Key Laboratory of Bengbu Medical College
ID : AHTT2022A002
Organisme : Open Topics of the Department level Key Laboratory of Bengbu Medical College
ID : AHTT2022A002
Organisme : Open Topics of the Department level Key Laboratory of Bengbu Medical College
ID : AHTT2022A002
Organisme : Open Topics of the Department level Key Laboratory of Bengbu Medical College
ID : AHTT2022A002
Organisme : Open Topics of the Department level Key Laboratory of Bengbu Medical College
ID : AHTT2022A002
Organisme : Postgraduate research innovation Program of Bengbu Medical College
ID : Byycx21071
Organisme : Postgraduate research innovation Program of Bengbu Medical College
ID : Byycx21071
Organisme : Postgraduate research innovation Program of Bengbu Medical College
ID : Byycx21071
Organisme : Postgraduate research innovation Program of Bengbu Medical College
ID : Byycx21071
Organisme : Postgraduate research innovation Program of Bengbu Medical College
ID : Byycx21071
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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