Structural and biochemical insight into a modular β-1,4-galactan synthase in plants.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
15
07
2022
accepted:
25
01
2023
pubmed:
1
3
2023
medline:
23
3
2023
entrez:
28
2
2023
Statut:
ppublish
Résumé
Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan synthase 1 (GalS1) transfers galactose and arabinose to either extend or cap the β-1,4-galactan side chains of RGI, respectively. Here we report the structure of GalS1 from Populus trichocarpa, showing a modular protein consisting of an N-terminal domain that represents the founding member of a new family of carbohydrate-binding module, CBM95, and a C-terminal glycosyltransferase family 92 (GT92) catalytic domain that adopts a GT-A fold. GalS1 exists as a dimer in vitro, with stem domains interacting across the chains in a 'handshake' orientation that is essential for maintaining stability and activity. In addition to understanding the enzymatic mechanism of GalS1, we gained insight into the donor and acceptor substrate binding sites using deep evolutionary analysis, molecular simulations and biochemical studies. Combining all the results, a mechanism for GalS1 catalysis and a new model for pectic galactan side-chain addition are proposed.
Identifiants
pubmed: 36849618
doi: 10.1038/s41477-023-01358-4
pii: 10.1038/s41477-023-01358-4
pmc: PMC10115243
mid: NIHMS1881134
doi:
Substances chimiques
Galactans
0
Glycosyltransferases
EC 2.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
486-500Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM124169
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103390
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM130915
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM139656
Pays : United States
Organisme : US Department of Energy
ID : DE-AC02-05CH11231
Organisme : NIGMS NIH HHS
ID : P30 GM124169
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM139656
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM130915
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Limited.
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