The D405N Mutation in the Spike Protein of SARS-CoV-2 Omicron BA.5 Inhibits Spike/Integrins Interaction and Viral Infection of Human Lung Microvascular Endothelial Cells.


Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
24 01 2023
Historique:
received: 20 12 2022
revised: 19 01 2023
accepted: 22 01 2023
entrez: 28 2 2023
pubmed: 1 3 2023
medline: 3 3 2023
Statut: epublish

Résumé

Severe COVID-19 is characterized by angiogenic features, such as intussusceptive angiogenesis, endothelialitis, and activation of procoagulant pathways. This pathological state can be ascribed to a direct SARS-CoV-2 infection of human lung ECs. Recently, we showed the capability of SARS-CoV-2 to infect ACE2-negative primary human lung microvascular endothelial cells (HL-mECs). This occurred through the interaction of an Arg-Gly-Asp (RGD) motif, endowed on the Spike protein at position 403-405, with α

Identifiants

pubmed: 36851546
pii: v15020332
doi: 10.3390/v15020332
pmc: PMC9962894
pii:
doi:

Substances chimiques

spike protein, SARS-CoV-2 0
Spike Glycoprotein, Coronavirus 0
Integrins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Antonella Bugatti (A)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

Federica Filippini (F)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

Serena Messali (S)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

Marta Giovanetti (M)

Laboratório de Flavivirus, Instituto Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil.
Department of Science and Technology for Humans and the Environment, University Campus Bio-Medico of Rome, 00128 Rome, Italy.

Cosetta Ravelli (C)

Section of General Pathology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

Alberto Zani (A)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

Massimo Ciccozzi (M)

Unit of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, 00128 Rome, Italy.

Arnaldo Caruso (A)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.
Institute of Human Virology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Francesca Caccuri (F)

Section of Microbiology, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.

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Classifications MeSH