L-serine biosynthesis in the human central nervous system: Structure and function of phosphoserine aminotransferase.
NMDA receptor
neurological disorders
phosphorylated pathway
phosphoserine aminotransferase
tumor progression factor
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
12
01
2023
received:
04
10
2022
accepted:
23
02
2023
medline:
3
4
2023
pubmed:
1
3
2023
entrez:
28
2
2023
Statut:
ppublish
Résumé
Organisms from all kingdoms of life synthesize L-serine (L-Ser) from 3-phosphoglycerate through the phosphorylated pathway, a three-step diversion of glycolysis. Phosphoserine aminotransferase (PSAT) catalyzes the intermediate step, the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and α-ketoglutarate. PSAT is particularly relevant in the central nervous system of mammals because L-Ser is the metabolic precursor of D-serine, cysteine, phospholipids, and nucleotides. Several mutations in the human psat gene have been linked to serine deficiency disorders, characterized by severe neurological symptoms. Furthermore, PSAT is overexpressed in many tumors and this overexpression has been associated with poor clinical outcomes. Here, we report the detailed functional and structural characterization of the recombinant human PSAT. The reaction catalyzed by PSAT is reversible, with an equilibrium constant of about 10, and the enzyme is very efficient, with a k
Identifiants
pubmed: 36851825
doi: 10.1002/pro.4609
pmc: PMC10031235
doi:
Substances chimiques
Phosphoglycerate Dehydrogenase
EC 1.1.1.95
phosphoserine aminotransferase
EC 2.6.1.52
Serine
452VLY9402
Transaminases
EC 2.6.1.-
PSAT1 protein, human
EC 2.6.1.52
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4609Informations de copyright
© 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.
Références
Arch Biochem Biophys. 1987 Apr;254(1):319-28
pubmed: 3579302
Eur J Dermatol. 2019 Dec 1;29(6):641-646
pubmed: 31903955
Arch Biochem Biophys. 2012 Mar 15;519(2):175-85
pubmed: 22023909
Appl Microbiol Biotechnol. 1995 Dec;44(1-2):27-36
pubmed: 8579834
Nucleic Acids Res. 2022 Jan 7;50(D1):D622-D631
pubmed: 34986597
Acta Crystallogr D Biol Crystallogr. 2012 May;68(Pt 5):553-63
pubmed: 22525753
Acta Crystallogr D Struct Biol. 2017 Feb 1;73(Pt 2):148-157
pubmed: 28177311
Arch Insect Biochem Physiol. 2019 Jun;101(2):e21553
pubmed: 31004387
Am J Hum Genet. 2007 May;80(5):931-7
pubmed: 17436247
J Biol Chem. 1960 Mar;235:620-4
pubmed: 14407079
Arch Biochem Biophys. 1981 Dec;212(2):717-29
pubmed: 7198894
Int J Biol Macromol. 2019 Jul 1;132:1012-1023
pubmed: 30959130
Life (Basel). 2021 May 26;11(6):
pubmed: 34073563
FEBS J. 2021 May;288(9):3034-3054
pubmed: 33249721
Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9
pubmed: 20457744
Sci Rep. 2017 May 18;7(1):2099
pubmed: 28522855
Biochem J. 1988 Nov 15;256(1):97-101
pubmed: 2851987
Oncotarget. 2017 Nov 6;8(61):104478-104491
pubmed: 29262655
J Inherit Metab Dis. 2020 Jul;43(4):758-769
pubmed: 32077105
FEBS Lett. 2013 Sep 2;587(17):2772-7
pubmed: 23892083
J Biol Chem. 1967 May 10;242(9):2283-7
pubmed: 6022873
Mol Biochem Parasitol. 2006 Jan;145(1):71-83
pubmed: 16289358
BMC Res Notes. 2010 Mar 03;3:52
pubmed: 20199659
Eur J Paediatr Neurol. 2016 Jan;20(1):53-60
pubmed: 26610677
J Enzyme Inhib Med Chem. 2013 Aug;28(4):879-93
pubmed: 22630075
Biochem J. 2009 Aug 13;422(2):265-72
pubmed: 19545238
Hum Mutat. 2020 Sep;41(9):1615-1628
pubmed: 32579715
Nat Commun. 2021 Jan 14;12(1):366
pubmed: 33446657
Biochim Biophys Acta. 1983 Dec 12;749(2):214-6
pubmed: 6652100
Am J Hum Genet. 2014 Sep 4;95(3):285-93
pubmed: 25152457
Protein Sci. 2005 Jun;14(6):1498-507
pubmed: 15883191
Amino Acids. 2012 Jul;43(1):483-91
pubmed: 22038178
J Biol Chem. 1959 May;234(5):1179-81
pubmed: 13654341
IUBMB Life. 1999 Nov;48(5):525-9
pubmed: 10637769
Biochim Biophys Acta. 1998 Jul 28;1386(1):29-38
pubmed: 9675237
Am J Med Genet A. 2021 Jul;185(7):2102-2107
pubmed: 34089226
Biochim Biophys Acta. 2016 Jul;1860(7):1508-18
pubmed: 27102280
J Med Chem. 2017 Feb 23;60(4):1227-1237
pubmed: 27959531
Protein Sci. 2023 Apr;32(4):e4609
pubmed: 36851825
Protein Sci. 2005 Jan;14(1):97-110
pubmed: 15608117
FEBS J. 2006 May;273(9):1920-9
pubmed: 16640556
Int J Mol Sci. 2021 Apr 19;22(8):
pubmed: 33921788
Curr Protoc Protein Sci. 2015 Feb 02;79:28.9.1-28.9.14
pubmed: 25640896
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Front Mol Biosci. 2019 Jan 09;5:112
pubmed: 30687716
Front Plant Sci. 2018 Jul 06;9:876
pubmed: 30034403
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Methods Enzymol. 1982;87:366-9
pubmed: 7176921
J Biol Chem. 2003 Mar 14;278(11):9481-8
pubmed: 12488449
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):293-302
pubmed: 21460447
Biochemistry. 2007 May 15;46(19):5810-8
pubmed: 17425331
Mol Genet Metab. 2018 Mar;123(3):309-316
pubmed: 29269105
Cell Physiol Biochem. 2016;39(1):395-406
pubmed: 27372650
Cell Mol Life Sci. 2020 Dec;77(24):5131-5148
pubmed: 32594192
J Mol Biol. 1999 Feb 26;286(3):829-50
pubmed: 10024454
Nucleic Acids Res. 2015 Jul 1;43(W1):W314-9
pubmed: 25908787
Biochem J. 2003 Jul 1;373(Pt 1):191-200
pubmed: 12633500
Nature. 2022 May;605(7911):747-753
pubmed: 35585241
Proteins. 2006 Jun 1;63(4):742-53
pubmed: 16532449
Biochemistry. 1998 Oct 27;37(43):15076-85
pubmed: 9790670
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Acta Crystallogr D Biol Crystallogr. 2013 May;69(Pt 5):804-11
pubmed: 23633589
Cancer Res. 2005 May 15;65(10):4101-17
pubmed: 15899800
Arch Biochem Biophys. 1995 May 10;319(1):49-54
pubmed: 7771805
Biomed Res Int. 2013;2013:161456
pubmed: 23781496
Adv Enzyme Regul. 1990;30:13-32
pubmed: 2119548
J Matern Fetal Neonatal Med. 2020 Mar;33(6):1033-1035
pubmed: 30122079
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2319-21
pubmed: 14646107
Cell Mol Life Sci. 2022 Jul 14;79(8):421
pubmed: 35834009
Eur Biophys J. 2011 May;40(5):599-610
pubmed: 21161522