Flow cytometry and immunohistochemistry of the mouse dural meninges for immunological and virological assessments.
Flow Cytometry/Mass Cytometry
Immunology
Microbiology
Microscopy
Model Organisms
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 03 2023
17 03 2023
Historique:
received:
21
12
2022
revised:
13
01
2023
accepted:
31
01
2023
medline:
23
10
2023
pubmed:
1
3
2023
entrez:
28
2
2023
Statut:
ppublish
Résumé
The highly vascularized meninges protect the surface of the central nervous system and contain a dense network of immune cells controlling neuroinfection and neuroinflammation. Here, we present techniques for the immunological and virological assessment of mouse dural meninges. We describe steps for immunophenotyping including meninges extraction and digestion, immunostaining, and flow cytometry. We then describe viral assessment upon lymphocytic choriomeningitis virus infection including steps for fixation of the meninges in the skull, whole-mount immunohistochemistry, and confocal imaging. For complete details on the use and execution of this protocol, please refer to Rebejac et al. (2022).
Identifiants
pubmed: 36853673
pii: S2666-1667(23)00077-1
doi: 10.1016/j.xpro.2023.102119
pmc: PMC9958090
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102119Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.