Notch3 regulates Mybl2 via HeyL to limit proliferation and tumor initiation in breast cancer.


Journal

Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092

Informations de publication

Date de publication:
28 02 2023
Historique:
received: 10 08 2022
accepted: 10 02 2023
revised: 08 02 2023
entrez: 28 2 2023
pubmed: 1 3 2023
medline: 3 3 2023
Statut: epublish

Résumé

Notch signaling is a conserved signaling pathway that participates in many aspects of mammary gland development and homeostasis, and has extensively been associated with breast tumorigenesis. Here, to unravel the as yet debated role of Notch3 in breast cancer development, we investigated its expression in human breast cancer samples and effects of its loss in mice. Notch3 expression was very weak in breast cancer cells and was associated with good patient prognosis. Interestingly, its expression was very strong in stromal cells of these patients, though this had no prognostic value. Mechanistically, we demonstrated that Notch3 prevents tumor initiation via HeyL-mediated inhibition of Mybl2, an important regulator of cell cycle. In the mammary glands of Notch3-deficient mice, we observed accelerated tumor initiation and proliferation in a MMTV-Neu model. Notch3-null tumors were enriched in Mybl2 mRNA signature and protein expression. Hence, our study reinforces the anti-tumoral role of Notch3 in breast tumorigenesis.

Identifiants

pubmed: 36854682
doi: 10.1038/s41419-023-05674-7
pii: 10.1038/s41419-023-05674-7
pmc: PMC9975231
doi:

Substances chimiques

Basic Helix-Loop-Helix Transcription Factors 0
Cell Cycle Proteins 0
HEYL protein, human 0
Heyl protein, mouse 0
MYBL2 protein, human 0
NOTCH3 protein, human 0
Receptor, Notch3 0
Repressor Proteins 0
Trans-Activators 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

171

Informations de copyright

© 2023. The Author(s).

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Auteurs

Sonia Brahim (S)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Ana-Maria Negulescu (AM)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Clara Geneste (C)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Thomas Schott (T)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Shuheng Lin (S)

Laboratory of Stem Cells and Cancer, Université Libre de Bruxelles, Brussels, Belgium.

Louis-Oscar Morel (LO)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Nicolas Rama (N)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.

Nicolas Gadot (N)

Centre Léon Bérard, Pathology Department, 69008, Lyon, France.

Isabelle Treilleux (I)

Centre Léon Bérard, Pathology Department, 69008, Lyon, France.

Patrick Mehlen (P)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France.
Centre Léon Bérard, Department of Translational Research and Innovation, 69008, Lyon, France.

Olivier Meurette (O)

Apoptosis, Cancer and Development Laboratory-Equipe labellisée 'La Ligue', LabEx DEVweCAN, Centre de Recherche en Cancérologie de Lyon, INSERM U1052-CNRS UMR5286, Université de Lyon, Centre Léon Bérard, 69008, Lyon, France. Olivier.meurette@lyon.unicancer.fr.

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Classifications MeSH