Determination of sex in cDNA samples from mice.

Animals Mice DNA, Complementary / genetics Retrospective Studies DNA / genetics Polymerase Chain Reaction / methods
Kdm5c Kdm5d brain cDNA development mouse sexing simplex PCR

Journal

BioTechniques
ISSN: 1940-9818
Titre abrégé: Biotechniques
Pays: England
ID NLM: 8306785

Informations de publication

Date de publication:
03 2023
Historique:
medline: 25 4 2023
pubmed: 2 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

The need to take sex into account in biomedical research is now recognized and mandated by funding institutions. In laboratory rodents, such as mice, sexing is usually performed anatomically or by genotyping using multiplex or simplex PCR techniques on genomic DNA. Here we present a simple RT-PCR-based method targeting

Identifiants

DOI: 10.2144/btn-2022-0077 PMID: 36856081
pubmed: 36856081
doi: 10.2144/btn-2022-0077
doi:

Substances chimiques

DNA, Complementary 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

149-152

Auteurs

Célia Bosso-Lefèvre (C)

Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, 75005 Paris, France.

Beetsi Urrieta-Chávez (B)

Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, 75005 Paris, France.

Fekrije Selimi (F)

Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, PSL Research University, 75005 Paris, France.
ORCID: 0000-0001-7704-5897

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Determination of sex in cDNA samples from mice.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Determination of sex in cDNA samples from mice.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › ADN complémentaire › Determination of sex in cDNA samples from mice.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études de cohortes › Études rétrospectives › Determination of sex in cDNA samples from mice.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Determination of sex in cDNA samples from mice.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Determination of sex in cDNA samples from mice.