Immunostaining of intact C. elegans using polyacrylamide embedding.

Animals Caenorhabditis elegans / chemistry Acrylic Resins Fixatives Antibodies
Antibody Cell Biology Developmental biology Microscopy Model Organisms Molecular Biology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 03 2023
Historique:
received: 05 09 2022
revised: 31 10 2022
accepted: 01 12 2022
medline: 23 10 2023
pubmed: 2 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

A major barrier to immunostaining Caenorhabditis elegans is the permeabilization of the worm's cuticle without distorting or damaging its body. We present here a gel-based immobilization protocol for fixed worms coupled with chemical and enzymatic permeabilization. The permeabilization is followed by antibody staining and fluorescent imaging. This protocol can be modified for different fixatives, permeabilizing reagents, or molecular readouts. Unlike previous immunostaining approaches, such as freeze cracking or dissection, this protocol enables immunostaining across the whole body of a well-preserved C. elegans.

Identifiants

DOI: 10.1016/j.xpro.2022.101956 PMID: 36856764 PMC: PMC9929481
pubmed: 36856764
pii: S2666-1667(22)00836-X
doi: 10.1016/j.xpro.2022.101956
pmc: PMC9929481
pii:
doi:

Substances chimiques

polyacrylamide 9003-05-8
Acrylic Resins 0
Fixatives 0
Antibodies 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101956

Informations de copyright

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Abbas Ghaddar (A)

University of Virginia, Department of Biology, Charlottesville, VA 22903, USA. Electronic address: ag2eu@virginia.edu.

Wenfan Ke (W)

University of Virginia, Department of Biology, Charlottesville, VA 22903, USA.

Eyleen J O'Rourke (EJ)

University of Virginia, Department of Biology, Charlottesville, VA 22903, USA; University of Virginia School of Medicine, Department of Cell Biology, Charlottesville, VA 22903, USA; Robert M. Berne Cardiovascular Research Center, School of Medicine, University of Virginia, Charlottesville, VA 22903, USA. Electronic address: ejorourke@virginia.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immunostaining of intact C. elegans using...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Caenorhabditis › Caenorhabditis elegans › Immunostaining of intact C. elegans using...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Résines synthétiques › Résines acryliques › Immunostaining of intact C. elegans using...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Fixateurs › Immunostaining of intact C. elegans using...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Immunostaining of intact C. elegans using...