Agarose native gel electrophoresis analysis of thermal aggregation controlled by Hofmeister series.

Sepharose Serum Albumin, Bovine / chemistry Thiocyanates Sodium Chloride / chemistry Tromethamine Electrophoresis, Agar Gel / methods
Agarose gel Hofmeister Mass photometry Native electrophoresis

Journal

Biophysical chemistry
ISSN: 1873-4200
Titre abrégé: Biophys Chem
Pays: Netherlands
ID NLM: 0403171

Informations de publication

Date de publication:
05 2023
Historique:
received: 23 11 2022
revised: 15 02 2023
accepted: 17 02 2023
medline: 7 4 2023
pubmed: 2 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

The effects of salting-in and salting-out salts defined by Hofmeister series on the solution state of bovine serum albumin (BSA) in 50 mM Tris-HCl buffer at pH 7.4 before and after thermal unfolding at 80 °C for 5 min were examined using agarose native gel electrophoresis and mass photometry. Gel electrophoresis showed that salting-in MgCl

Identifiants

DOI: 10.1016/j.bpc.2023.106977 PMID: 36857888
pubmed: 36857888
pii: S0301-4622(23)00028-5
doi: 10.1016/j.bpc.2023.106977
pii:
doi:

Substances chimiques

sodium thiocyanate 5W0K9HKA05
Sepharose 9012-36-6
Serum Albumin, Bovine 27432CM55Q
Thiocyanates 0
Sodium Chloride 451W47IQ8X
Tromethamine 023C2WHX2V

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

106977

Informations de copyright

Copyright © 2023 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest This work was supported by Kyokuto Pharmaceutical Industrial Co., Ltd. and Refeyn Japan. Y.T., R.S., and T.A. (Teruo Akuta) are employees of the for-profit company Kyokuto Pharmaceuticals. R.T. and K.S. are emplyees of the for-profit company Refeyn Japan, K.K. S.N and K.T. are staffs of University of Tokyo and have no conflict of interest. Tsutomu Arakawa used to belong to the for-profit company Alliance Protein Laboratories but currently has no conflict of interest.

Auteurs

Yui Tomioka (Y)

Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan. Electronic address: y.tomioka@kyokutoseiyaku.co.jp.

Ryo Sato (R)

Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan. Electronic address: r.satou2@kyokutoseiyaku.co.jp.

Ryo Takahashi (R)

Refeyn Japan, K.K., 1-1-14, Sakuraguchi-cho, Nada-ku, Kobe, Hyogo 6570036, Japan. Electronic address: ryo.takahashi@refeyn.com.

Satoru Nagatoishi (S)

The Institute of Medical Sciences, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Electronic address: nagatoishi@bioeng.t.u-tokyo.ac.jp.

Kohei Shiba (K)

Refeyn Japan, K.K., 1-1-14, Sakuraguchi-cho, Nada-ku, Kobe, Hyogo 6570036, Japan. Electronic address: kohei.shiba@refeyn.com.

Kouhei Tsumoto (K)

The Institute of Medical Sciences, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: tsumoto@bioeng.t.u-tokyo.ac.jp.

Tsutomu Arakawa (T)

Alliance Protein Laboratories, 13380 Pantera Rd, San Diego, CA 92130, USA. Electronic address: tarakawa2@aol.com.

Teruo Akuta (T)

Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan. Electronic address: t.akuta@kyokutoseiyaku.co.jp.

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Classifications MeSH

questionsmedicales.fr › Glucides › Polyosides › Agarose › Agarose native gel electrophoresis analysis of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du sang › Sérumalbumine › Sérumalbumine bovine › Agarose native gel electrophoresis analysis of...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Thiocyanates › Agarose native gel electrophoresis analysis of...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du sodium › Chlorure de sodium › Agarose native gel electrophoresis analysis of...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Glycols › Propylène glycols › Trométhamine › Agarose native gel electrophoresis analysis of...
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel d'agar › Agarose native gel electrophoresis analysis of...