Single-cell analysis reveals dynamics of human B cell differentiation and identifies novel B and antibody-secreting cell intermediates.
B cell
antibody-secreting cell
cell biology
differentiation
human
immunology
inflammation
single-cell RNA sequencing
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
02 03 2023
02 03 2023
Historique:
received:
20
09
2022
accepted:
24
02
2023
pubmed:
3
3
2023
medline:
15
3
2023
entrez:
2
3
2023
Statut:
epublish
Résumé
Differentiation of B cells into antibody-secreting cells (ASCs) is a key process to generate protective humoral immunity. A detailed understanding of the cues controlling ASC differentiation is important to devise strategies to modulate antibody formation. Here, we dissected differentiation trajectories of human naive B cells into ASCs using single-cell RNA sequencing. By comparing transcriptomes of B cells at different stages of differentiation from an in vitro model with ex vivo B cells and ASCs, we uncovered a novel pre-ASC population present ex vivo in lymphoid tissues. For the first time, a germinal-center-like population is identified in vitro from human naive B cells and possibly progresses into a memory B cell population through an alternative route of differentiation, thus recapitulating in vivo human GC reactions. Our work allows further detailed characterization of human B cell differentiation into ASCs or memory B cells in both healthy and diseased conditions.
Identifiants
pubmed: 36861964
doi: 10.7554/eLife.83578
pii: 83578
pmc: PMC10005767
doi:
pii:
Banques de données
GEO
['GSE214265', 'GSE12366', 'GSE137275']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023, Verstegen, Pollastro et al.
Déclaration de conflit d'intérêts
NV, SP, PU, CM, GE, TJ, MS, KB, KH, TR, JS, At, MB, Sv No competing interests declared
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