O-GlcNAc glycosylation orchestrates fate decision and niche function of bone marrow stromal progenitors.
biochemistry
bone
bone marrow
chemical biology
developmental biology
hematopoiesis
mouse
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
02 03 2023
02 03 2023
Historique:
received:
08
12
2022
accepted:
01
03
2023
pubmed:
3
3
2023
medline:
25
3
2023
entrez:
2
3
2023
Statut:
epublish
Résumé
In mammals, interactions between the bone marrow (BM) stroma and hematopoietic progenitors contribute to bone-BM homeostasis. Perinatal bone growth and ossification provide a microenvironment for the transition to definitive hematopoiesis; however, mechanisms and interactions orchestrating the development of skeletal and hematopoietic systems remain largely unknown. Here, we establish intracellular O-linked β-N-acetylglucosamine (O-GlcNAc) modification as a posttranslational switch that dictates the differentiation fate and niche function of early BM stromal cells (BMSCs). By modifying and activating RUNX2, O-GlcNAcylation promotes osteogenic differentiation of BMSCs and stromal IL-7 expression to support lymphopoiesis. In contrast, C/EBPβ-dependent marrow adipogenesis and expression of myelopoietic stem cell factor (SCF) is inhibited by O-GlcNAcylation. Ablating O-GlcNAc transferase (OGT) in BMSCs leads to impaired bone formation, increased marrow adiposity, as well as defective B-cell lymphopoiesis and myeloid overproduction in mice. Thus, the balance of osteogenic and adipogenic differentiation of BMSCs is determined by reciprocal O-GlcNAc regulation of transcription factors, which simultaneously shapes the hematopoietic niche.
Identifiants
pubmed: 36861967
doi: 10.7554/eLife.85464
pii: 85464
pmc: PMC10032655
doi:
pii:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI162791
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI139420
Pays : United States
Organisme : NIH HHS
ID : R01 AI139420
Pays : United States
Organisme : NIH HHS
ID : R01 AI162791
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI162791
Pays : United States
Organisme : NIH HHS
ID : R01 AI162678
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2023, Zhang, Huang et al.
Déclaration de conflit d'intérêts
ZZ, ZH, MA, ME, JC, LB, JM, AB, HZ, KM, HR No competing interests declared
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