A succession of two viral lattices drives vaccinia virus assembly.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
27
10
2022
accepted:
19
01
2023
revised:
14
03
2023
pubmed:
3
3
2023
medline:
17
3
2023
entrez:
2
3
2023
Statut:
epublish
Résumé
During its cytoplasmic replication, vaccinia virus assembles non-infectious spherical immature virions (IV) coated by a viral D13 lattice. Subsequently, IV mature into infectious brick-shaped intracellular mature virions (IMV) that lack D13. Here, we performed cryo-electron tomography (cryo-ET) of frozen-hydrated vaccinia-infected cells to structurally characterise the maturation process in situ. During IMV formation, a new viral core forms inside IV with a wall consisting of trimeric pillars arranged in a new pseudohexagonal lattice. This lattice appears as a palisade in cross-section. As maturation occurs, which involves a 50% reduction in particle volume, the viral membrane becomes corrugated as it adapts to the newly formed viral core in a process that does not appear to require membrane removal. Our study suggests that the length of this core is determined by the D13 lattice and that the consecutive D13 and palisade lattices control virion shape and dimensions during vaccinia assembly and maturation.
Identifiants
pubmed: 36862727
doi: 10.1371/journal.pbio.3002005
pii: PBIOLOGY-D-22-02402
pmc: PMC10013923
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3002005Subventions
Organisme : Cancer Research UK
ID : CC2096
Pays : United Kingdom
Organisme : Medical Research Council
ID : CC2096
Pays : United Kingdom
Organisme : Wellcome Trust
ID : CC2096
Pays : United Kingdom
Organisme : Cancer Research UK
ID : CC2106
Pays : United Kingdom
Organisme : Medical Research Council
ID : CC2106
Pays : United Kingdom
Organisme : Wellcome Trust
ID : CC2106
Pays : United Kingdom
Informations de copyright
Copyright: © 2023 Hernandez-Gonzalez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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