First-in-class Microbial Ecosystem Therapeutic 4 (MET4) in combination with immune checkpoint inhibitors in patients with advanced solid tumors (MET4-IO trial).

advanced solid tumors first in class microbial ecosystem therapeutic 4 immune checkpoint inhibitors intestinal microbiome

Journal

Annals of oncology : official journal of the European Society for Medical Oncology
ISSN: 1569-8041
Titre abrégé: Ann Oncol
Pays: England
ID NLM: 9007735

Informations de publication

Date de publication:
06 2023
Historique:
received: 11 12 2022
revised: 16 02 2023
accepted: 21 02 2023
medline: 12 6 2023
pubmed: 3 3 2023
entrez: 2 3 2023
Statut: ppublish

Résumé

The intestinal microbiome has been associated with response to immune checkpoint inhibitors (ICIs) in humans and causally implicated in ICI responsiveness in animal models. Two recent human trials demonstrated that fecal microbiota transplant (FMT) from ICI responders can rescue ICI responses in refractory melanoma, but FMT has specific limitations to scaled use. We conducted an early-phase clinical trial of a cultivated, orally delivered 30-species microbial consortium (Microbial Ecosystem Therapeutic 4, MET4) designed for co-administration with ICIs as an alternative to FMT and assessed safety, tolerability and ecological responses in patients with advanced solid tumors. The trial achieved its primary safety and tolerability outcomes. There were no statistically significant differences in the primary ecological outcomes; however, differences in MET4 species relative abundance were evident after randomization that varied by patient and species. Increases in the relative abundance of several MET4 taxa, including Enterococcus and Bifidobacterium, taxa previously associated with ICI responsiveness, were observed and MET4 engraftment was associated with decreases in plasma and stool primary bile acids. This trial is the first report of the use of a microbial consortium as an alternative to FMT in advanced cancer patients receiving ICI and the results justify the further development of microbial consortia as a therapeutic co-intervention for ICI treatment in cancer.

Sections du résumé

BACKGROUND
The intestinal microbiome has been associated with response to immune checkpoint inhibitors (ICIs) in humans and causally implicated in ICI responsiveness in animal models. Two recent human trials demonstrated that fecal microbiota transplant (FMT) from ICI responders can rescue ICI responses in refractory melanoma, but FMT has specific limitations to scaled use.
PATIENTS AND METHODS
We conducted an early-phase clinical trial of a cultivated, orally delivered 30-species microbial consortium (Microbial Ecosystem Therapeutic 4, MET4) designed for co-administration with ICIs as an alternative to FMT and assessed safety, tolerability and ecological responses in patients with advanced solid tumors.
RESULTS
The trial achieved its primary safety and tolerability outcomes. There were no statistically significant differences in the primary ecological outcomes; however, differences in MET4 species relative abundance were evident after randomization that varied by patient and species. Increases in the relative abundance of several MET4 taxa, including Enterococcus and Bifidobacterium, taxa previously associated with ICI responsiveness, were observed and MET4 engraftment was associated with decreases in plasma and stool primary bile acids.
CONCLUSIONS
This trial is the first report of the use of a microbial consortium as an alternative to FMT in advanced cancer patients receiving ICI and the results justify the further development of microbial consortia as a therapeutic co-intervention for ICI treatment in cancer.

Identifiants

pubmed: 36863483
pii: S0923-7534(23)00088-1
doi: 10.1016/j.annonc.2023.02.011
pii:
doi:

Substances chimiques

Immune Checkpoint Inhibitors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

520-530

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Auteurs

A Spreafico (A)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto; Tumor Immunotherapy Program, Princess Margaret Cancer Centre, University Health Network, Toronto. Electronic address: anna.spreafico@uhn.ca.

A A Heirali (AA)

Toronto General Hospital Research Institute, University Health Network, Toronto, Canada.

D V Araujo (DV)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto; Department of Medical Oncology, Hospital de Base, Sao Paulo, Brazil.

T J Tan (TJ)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto; Division of Medical Oncology, National Cancer Centre Singapore, Singapore, Singapore.

M Oliva (M)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto; Department of Medical Oncology, Institut Catala d' Oncologia, L'Hospitalet de Llobregat, Barcelona; Universitat de Barcelona, Barcelona, Spain.

P H H Schneeberger (PHH)

Toronto General Hospital Research Institute, University Health Network, Toronto, Canada; Swiss Tropical and Public Health Institute, Department of Medical Parasitology and Infection Biology, Allschwil; University of Basel, Basel, Switzerland.

B Chen (B)

Biostatistics Department, Princess Margaret Cancer Centre, University Health Network, Toronto.

M K Wong (MK)

Department of Immunology, University of Toronto, Toronto.

L-A Stayner (LA)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto.

A R Hansen (AR)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto.

S D Saibil (SD)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto.

B X Wang (BX)

Tumor Immunotherapy Program, Princess Margaret Cancer Centre, University Health Network, Toronto.

K Cochrane (K)

Nubiyota LLP, Guelph, Canada.

K Sherriff (K)

Nubiyota LLP, Guelph, Canada.

E Allen-Vercoe (E)

Nubiyota LLP, Guelph, Canada.

W Xu (W)

Biostatistics Department, Princess Margaret Cancer Centre, University Health Network, Toronto.

L L Siu (LL)

Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto; Tumor Immunotherapy Program, Princess Margaret Cancer Centre, University Health Network, Toronto.

B Coburn (B)

Toronto General Hospital Research Institute, University Health Network, Toronto, Canada. Electronic address: Bryan.Coburn@uhn.ca.

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