Downregulation of KEAP1 in melanoma promotes resistance to immune checkpoint blockade.


Journal

NPJ precision oncology
ISSN: 2397-768X
Titre abrégé: NPJ Precis Oncol
Pays: England
ID NLM: 101708166

Informations de publication

Date de publication:
02 Mar 2023
Historique:
received: 25 08 2022
accepted: 10 02 2023
entrez: 2 3 2023
pubmed: 3 3 2023
medline: 3 3 2023
Statut: epublish

Résumé

Immune checkpoint blockade (ICB) has demonstrated efficacy in patients with melanoma, but many exhibit poor responses. Using single cell RNA sequencing of melanoma patient-derived circulating tumor cells (CTCs) and functional characterization using mouse melanoma models, we show that the KEAP1/NRF2 pathway modulates sensitivity to ICB, independently of tumorigenesis. The NRF2 negative regulator, KEAP1, shows intrinsic variation in expression, leading to tumor heterogeneity and subclonal resistance.

Identifiants

pubmed: 36864091
doi: 10.1038/s41698-023-00362-3
pii: 10.1038/s41698-023-00362-3
pmc: PMC9981575
doi:

Types de publication

Journal Article

Langues

eng

Pagination

25

Subventions

Organisme : NCI NIH HHS
ID : R01 CA129933
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007753
Pays : United States

Informations de copyright

© 2023. The Author(s).

Références

Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
N Engl J Med. 2014 Dec 4;371(23):2189-2199
pubmed: 25409260
Nat Methods. 2015 May;12(5):453-7
pubmed: 25822800
Nature. 2014 Nov 27;515(7528):568-71
pubmed: 25428505
Cancer Res. 2013 Apr 1;73(7):2199-210
pubmed: 23382044
Nat Protoc. 2017 Mar;12(3):534-547
pubmed: 28182018
Cell Syst. 2015 Dec 23;1(6):417-425
pubmed: 26771021
Nat Methods. 2014 Aug;11(8):783-784
pubmed: 25075903
N Engl J Med. 2015 Jul 2;373(1):23-34
pubmed: 26027431
Science. 2013 Feb 1;339(6119):580-4
pubmed: 23372014
Nat Immunol. 2019 Dec;20(12):1574-1583
pubmed: 31745335
Nat Protoc. 2014 Mar;9(3):694-710
pubmed: 24577360
J Clin Invest. 2016 Sep 1;126(9):3447-52
pubmed: 27525433
N Engl J Med. 2016 Sep 1;375(9):819-29
pubmed: 27433843
Sci Transl Med. 2013 Apr 3;5(179):179ra47
pubmed: 23552373
Nat Protoc. 2014 Jan;9(1):171-81
pubmed: 24385147
Nat Med. 2017 Nov;23(11):1362-1368
pubmed: 28967920
Cancer Res. 1975 Jan;35(1):218-24
pubmed: 1109790
Cancer Cell. 2018 Jul 9;34(1):21-43
pubmed: 29731393
Cell Res. 2016 Mar;26(3):304-19
pubmed: 26902283
Nat Commun. 2017 Oct 26;8(1):1136
pubmed: 29070816
Nat Rev Immunol. 2020 Jan;20(1):25-39
pubmed: 31570880
Science. 2014 Jan 3;343(6166):84-87
pubmed: 24336571
Nat Immunol. 2019 Mar;20(3):326-336
pubmed: 30778252
Nat Biotechnol. 2016 Feb;34(2):184-191
pubmed: 26780180
Cancer Discov. 2020 Sep;10(9):1296-1311
pubmed: 32371478
Science. 2018 Nov 30;362(6418):1060-1063
pubmed: 30498128
Nat Med. 2019 Dec;25(12):1916-1927
pubmed: 31792460
Nat Protoc. 2012 Mar 01;7(3):562-78
pubmed: 22383036
Cancer Discov. 2021 Mar;11(3):678-695
pubmed: 33203734
PLoS One. 2008;3(11):e3647
pubmed: 18985154
Sci Rep. 2019 Mar 20;9(1):4902
pubmed: 30894629
Clin Cancer Res. 2016 Mar 1;22(5):1103-10
pubmed: 26446944
Science. 2014 Jul 11;345(6193):216-20
pubmed: 25013076
Science. 2015 Sep 18;349(6254):1351-6
pubmed: 26383955
Bioinformatics. 2011 Jun 1;27(11):1571-2
pubmed: 21493656
N Engl J Med. 2015 Jun 25;372(26):2521-32
pubmed: 25891173
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Science. 2018 Mar 23;359(6382):1350-1355
pubmed: 29567705

Auteurs

Douglas B Fox (DB)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Richard Y Ebright (RY)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Xin Hong (X)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Department of Biochemistry, School of Medicine and Key University Laboratory of Metabolism and Health of Guangdong, Southern University of Science and Technology, Shenzhen, 518055, China.

Hunter C Russell (HC)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Hongshan Guo (H)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Liangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, 311121, China.

Thomas J LaSalle (TJ)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Broad Institute of Harvard and MIT, Cambridge, MA, 02139, USA.

Ben S Wittner (BS)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Nicolas Poux (N)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Joanna A Vuille (JA)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.

Mehmet Toner (M)

Center for Engineering in Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.
Shriners Hospitals for Children, Boston, MA, 02114, USA.
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Nir Hacohen (N)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Broad Institute of Harvard and MIT, Cambridge, MA, 02139, USA.

Genevieve M Boland (GM)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Debattama R Sen (DR)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Ryan J Sullivan (RJ)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA.
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA.

Shyamala Maheswaran (S)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA. Maheswaran@helix.mgh.harvard.edu.
Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA. Maheswaran@helix.mgh.harvard.edu.

Daniel A Haber (DA)

Massachusetts General Hospital Cancer Center and Harvard Medical School, Boston, MA, 02114, USA. Dhaber@mgh.harvard.edu.
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA. Dhaber@mgh.harvard.edu.
Howard Hughes Medical Institute, Bethesda, MD, 20815, USA. Dhaber@mgh.harvard.edu.

Classifications MeSH